Patents by Inventor Tsuyoshi Nakamatsu
Tsuyoshi Nakamatsu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8183017Abstract: The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.Type: GrantFiled: October 29, 2010Date of Patent: May 22, 2012Assignee: Ajinomoto Co., Inc.Inventors: Seiko Otsuna, Masakazu Sugimoto, Masako Izui, Atsushi Hayakawa, Eiichi Nakano, Masaki Kobayashi, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
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Publication number: 20110065153Abstract: The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.Type: ApplicationFiled: October 29, 2010Publication date: March 17, 2011Inventors: SEIKO OTSUNA, MASAKAZU SUGIMOTO, MASAKO IZUI, ATSUSHI HAYAKAWA, EIICHI NAKANO, MASAKI KOBAYASHI, YASUHIKO YOSHIHARA, TSUYOSHI NAKAMATSU
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Patent number: 7858348Abstract: The present invention provides genes that encode the N-acetyl-(R,S)-?-amino acid acylases. The N-acetyl-(R,S)-?-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-?-amino acid acylase produced by Variovorax sp.Type: GrantFiled: July 24, 2007Date of Patent: December 28, 2010Assignee: Ajinomoto Co., Inc.Inventors: Shunichi Suzuki, Yuki Imabayashi, Kunihiko Watanabe, Hisashi Kawasaki, Tsuyoshi Nakamatsu
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Patent number: 7846698Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.Type: GrantFiled: August 23, 2002Date of Patent: December 7, 2010Assignee: Ajinomoto Co., Inc.Inventors: Seiko Otsuna, Masakazu Sugimoto, Masako Izui, Atsushi Hayakawa, Eiichi Nakano, Masaki Kobayashi, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
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Patent number: 7833760Abstract: The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an S-?-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a process for the selective production of an S-, or R-?-amino acid using the above strains.Type: GrantFiled: October 26, 2007Date of Patent: November 16, 2010Assignee: Ajinomoto Co., Inc.Inventors: Tsuyoshi Nakamatsu, Hisashi Kawasaki, Kunihiko Watanabe, Masakazu Nakazawa, Kunisuke Izawa
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Publication number: 20100124777Abstract: L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting a gene coding for trehalose-6-phosphate synthase, a gene coding for maltooligosyltrehalose synthase, or both of these genes to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.Type: ApplicationFiled: April 19, 2007Publication date: May 20, 2010Applicant: Ajinomoto Co., Inc.Inventors: Hiromi Ohtaki, Jun Nakamura, Hiroshi Izui, Tsuyoshi Nakamatsu
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Patent number: 7608437Abstract: A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.Type: GrantFiled: September 6, 2005Date of Patent: October 27, 2009Assignee: Ajinomoto Co., Inc.Inventors: Yoko Asakura, Jun Nakamura, Sohei Kanno, Mikiko Suga, Eiichiro Kimura, Hisao Ito, Kazuhiko Matsui, Tsuyoshi Ohsumi, Tsuyoshi Nakamatsu, Osamu Kurahashi
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Publication number: 20090258398Abstract: The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an S-?-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a process for the selective production of an S-, or R-?-amino acid using the above strains.Type: ApplicationFiled: October 26, 2007Publication date: October 15, 2009Inventors: Tsuyoshi Nakamatsu, Hisashi Kawasaki, Kunihiko Watanabe, Masakazu Nakazawa, Kunisuke Izawa
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Patent number: 7432085Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: November 18, 2005Date of Patent: October 7, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Publication number: 20080241895Abstract: The present invention provides genes that encode the N-acetyl-(R,S)-?-amino acid acylases. The N-acetyl-(R,S)-?-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-?-amino acid acylase produced by Variovorax sp.Type: ApplicationFiled: July 24, 2007Publication date: October 2, 2008Inventors: Shunichi Suzuki, Yuki Imabayashi, Kunihiko Watanabe, Hisashi Kawasaki, Tsuyoshi Nakamatsu
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Patent number: 7351571Abstract: The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an S-?-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a process for the selective production of an S-, or R-?-amino acid using the above strains.Type: GrantFiled: August 8, 2005Date of Patent: April 1, 2008Assignee: Ajinomoto Co., Inc.Inventors: Tsuyoshi Nakamatsu, Hisashi Kawasaki, Kunihiko Watanabe, Masakazu Nakazawa, Kunisuke Izawa
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Patent number: 7307160Abstract: L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting the otsA gene derived from a coryneform bacterium source, coding for trehalose-6-phosphate synthase, to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.Type: GrantFiled: August 11, 2003Date of Patent: December 11, 2007Assignee: Ajinomoto Co. Inc.Inventors: Hiromi Ohtaki, Jun Nakamura, Hiroshi Izui, Tsuyoshi Nakamatsu
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Patent number: 7297521Abstract: A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carType: GrantFiled: December 15, 2004Date of Patent: November 20, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yoko Kuwabara, Kenichi Hashiguchi, Tsuyoshi Nakamatsu, Osamu Kurahashi, Yukiko Mori, Hisao Ito
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Patent number: 7262035Abstract: L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.Type: GrantFiled: February 5, 2002Date of Patent: August 28, 2007Assignee: Ajinomoto Co., Inc.Inventors: Jun Nakamura, Hiroshi Izui, Kayo Moriguchi, Hiroki Kawashima, Tsuyoshi Nakamatsu, Osamu Kurahashi
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Patent number: 7244569Abstract: A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.Type: GrantFiled: April 27, 2004Date of Patent: July 17, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yumi Matsuzaki, Eiichiro Kimura, Tsuyoshi Nakamatsu, Osamu Kurahashi, Yoshio Kawahara, Shinichi Sugimoto
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Publication number: 20070092953Abstract: L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.Type: ApplicationFiled: December 27, 2006Publication date: April 26, 2007Applicant: AJINOMOTO CO. INCInventors: Jun Nakamura, Hiroshi Izui, Kayo Moriguchi, Hiroki Kawashima, Tsuyoshi Nakamatsu, Osamu Kurahashi
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Patent number: 7183403Abstract: A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.Type: GrantFiled: March 8, 2005Date of Patent: February 27, 2007Assignee: Ajinomoto Co., Inc.Inventors: Seiko Hirano, Eiichiro Kimura, Tsuyoshi Osumi, Kazuhiko Matsui, Yoshio Kawahara, Gen Nonaka, Yumi Matsuzaki, Naoki Akiyoshi, Kanae Nakamura, Osamu Kurahashi, Tsuyoshi Nakamatsu, Shinichi Sugimoto
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Patent number: 7125977Abstract: A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.Type: GrantFiled: March 8, 2005Date of Patent: October 24, 2006Assignee: Ajinomoto Co., Inc.Inventors: Seiko Hirano, Eiichiro Kimura, Tsuyoshi Osumi, Kazuhiko Matsui, Yoshio Kawahara, Gen Nonaka, Yumi Matsuzaki, Naoki Akiyoshi, Kanae Nakamura, Osamu Kurahashi, Tsuyoshi Nakamatsu, Shinichi Sugimoto
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Publication number: 20060115878Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: ApplicationFiled: November 18, 2005Publication date: June 1, 2006Applicant: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Patent number: 7037690Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: March 26, 2003Date of Patent: May 2, 2006Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu