Abstract: A discharge member with a filter enables a filtrate to be dripped into a dripping container while filtering a suspension, and enables an excessive portion to be suctioned when the dripped amount is excessive, thereby to allow a fixed amount of a filtrate to be injected into the dripping container. The discharge member may enable suctioning operation at the time of suctioning an excessive portion to be conducted without deteriorating its dripping performance and filtering performance. The filter provided in the discharge member is a composite filter in which a pre-filter including a continuous porous foam that is softened at least in a wet state is arranged on a filtration surface side of a membrane filter.

Abstract: A discharge member with a filter enables a filtrate to be dripped into a dripping container while filtering a suspension, and enables an excessive portion to be suctioned when the dripped amount is excessive, thereby to allow a fixed amount of a filtrate to be injected into the dripping container. The discharge member may enable suctioning operation at the time of suctioning an excessive portion to be conducted without deteriorating its dripping performance and filtering performance. The filter provided in the discharge member is a composite filter in which a pre-filter including a continuous porous foam that is softened at least in a wet state is arranged on a filtration surface side of a membrane filter.

Abstract: The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.

Abstract: The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.