Patents by Inventor Tsuyoshi Shirai

Tsuyoshi Shirai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • HEAT RESISTANT MISMATCH ENDONUCLEASE VARIANT
    Publication number: 20230133071
    Abstract: The present invention provides a GG-specific mismatch endonuclease variant, a TT-specific mismatch endonuclease variant, and a GT/TG-specific mismatch endonuclease variant. The present invention also provides a mismatch specific cleaving reaction using said variant, a method for removing errors in a nucleic acid amplification reaction using a mismatch nuclease, a method for suppressing amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single base polymorphic mutation using said suppression method.
    Type: Application
    Filed: March 18, 2021
    Publication date: May 4, 2023
    Applicants: TAKARA BIO INC., EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUEN, KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventors: Hiroyuki MATSUMOTO, Takashi UEMORI, Tsuyoshi SHIRAI, Yoshizumi ISHINO, Sonoko ISHINO
  • INFLAMMATORY BOWEL DISEASE DIAGNOSIS METHOD, DIAGNOSIS PROBE AND DIAGNOSIS KIT
    Publication number: 20220276243
    Abstract: Provided are a novel determination method, diagnostic probe, and diagnostic kit for inflammatory bowel disease. A method for determining inflammatory bowel disease includes measuring an anti-endothelial cell protein C receptor antibody (anti-EPCR antibody) in a sample collected from a subject.
    Type: Application
    Filed: August 25, 2020
    Publication date: September 1, 2022
    Applicant: TOHOKU UNIVERSITY
    Inventors: Tsuyoshi SHIRAI, Hiroshi FUJII, Tomonori ISHII, Hideo HARIGAE, Yoichi KAKUTA, Atsushi MASAMUNE, Tomoyuki MUTOH
  • Methods of utilizing thermostable mismatch endonuclease
    Patent number: 10975415
    Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.
    Type: Grant
    Filed: September 9, 2015
    Date of Patent: April 13, 2021
    Assignees: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUEN
    Inventors: Takashi Uemori, Yoshizumi Ishino, Takehiro Sagara, Sonoko Ishino, Takeshi Yamagami, Tsuyoshi Shirai
  • METHODS OF UTILIZING THERMOSTABLE MISMATCH ENDONUCLEASE
    Publication number: 20170253909
    Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.
    Type: Application
    Filed: September 9, 2015
    Publication date: September 7, 2017
    Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUEN
    Inventors: Takashi UEMORI, Yoshizumi ISHINO, Takehiro SAGARA, Sonoko ISHINO, Takeshi YAMAGAMI, Tsuyoshi SHIRAI
  • Remote control device for vehicle
    Patent number: 7332997
    Abstract: A door lock drive controller, after outputting a drive signal to a drive circuit of a door lock actuator based on a signal from a remote control terminal, outputs an answer back signal in the form of pulses to a lamp drive circuit to light a hazard lamp. In a buzzer drive controller, pulses are converted to waveforms with changing voltage values at a waveform converter. A repetitive pulse with frequencies that changes is generated by a frequency generator based on the waveform-converted pulses and, in accordance therewith, a buzzer is driven by an output unit. A whistle-like tone can be obtained by causing the frequency of the repetitive pulse to change in the direction of increase.
    Type: Grant
    Filed: December 8, 2004
    Date of Patent: February 19, 2008
    Assignee: Niles Co., Ltd.
    Inventors: Tsuyoshi Shirai, Seizi Watanabe
  • Remote control device for vehicle
    Publication number: 20050212651
    Abstract: A door lock drive controller, after outputting a drive signal to a drive circuit of a door lock actuator based on a signal from a remote control terminal, outputs an answer back signal in the form of pulses to a lamp drive circuit to light a hazard lamp. In a buzzer drive controller, pulses are converted to waveforms with changing voltage values at a waveform converter. A repetitive pulse with frequencies that changes is generated by a frequency generator based on the waveform-converted pulses and, in accordance therewith, a buzzer is driven by an output unit. A whistle-like tone can be obtained by causing the frequency of the repetitive pulse to change in the direction of increase.
    Type: Application
    Filed: December 8, 2004
    Publication date: September 29, 2005
    Inventors: Tsuyoshi Shirai, Seizi Watanabe