Abstract: A fluorescent detection apparatus relates to an analysis technique for qualitatively detecting or quantifying biomolecules by producing an evanescent field on a surface of a substrate, exciting fluorescently labelled biomolecules on the substrate surface in the evanescent field, and detecting the resultant fluorescent light emitted from the biomolecules. The fluorescent detection apparatus has a configuration in which a well is provided in a surface opposing to a sample substrate of a prism, the well is filled with a matching liquid, and the matching liquid is filled between the sample substrate and the prism, thereby improving operability and providing a stable evanescent field.

Abstract: An electrophoresis member is produced by laying a plurality of capillaries on an adhesive layer born on a support layer to form a capillary layer, laminating thereon a second support layer, and partially removing the first support layer, the first adhesive layer and the second support layer to partially expose the capillaries to form a window portion for irradiation and detection and a sample injection portion for injecting a sample.

Abstract: A fluorescent detection apparatus relates to an analysis technique for qualitatively detecting or quantifying biomolecules by producing an evanescent field on a surface of a substrate, exciting fluorescently labelled biomolecules on the substrate surface in the evanescent field, and detecting the resultant fluorescent light emitted from the biomolecules. The fluorescent detection apparatus has a configuration in which a well is provided in a surface opposing to a sample substrate of a prism, the well is filled with a matching liquid, and the matching liquid is filled between the sample substrate and the prism, thereby improving operability and providing a stable evanescent field.

Abstract: A metallic structure is provided on a surface of a substrate. A component having a longer wavelength than excitation light is detected from luminescence from fixation positions of biomolecules and emitted from a material other than the biomolecules, and is used for photometrical analysis. As the structure, usable is a particulate (a metallic structure of a size not larger than a wavelength of the excitation light), a minute protrusion, or a thin film with minute apertures, which are made of a metal such as gold, chrome, silver or aluminum. In the case of the particulate or the minute protrusion, photoluminescence of the structure is detected with a biomolecule being fixed thereon. In the case of the thin film with minute apertures, Raman scattered light of specimen solution around the biomolecules, and photoluminescence of the metallic structure near the biomolecules are detected with biomolecules being fixed in the apertures.

Abstract: Single molecule measurement is conducted by propagating a laser light under multiple reflection between two substrates constituting a flow channel for flowing a sample solution, exciting target molecules in the sample, detecting one-dimensional images of generated fluorescence by a one-dimensional detection portion, synthesizing two-dimensional images from the obtained one-dimensional images by a data synthesizing portion and measuring the concentration of target molecules in the sample solution by counting the number of target molecules based on the two-dimensional images, thereby measuring the concentration of the target molecules in the sample solution. These constitutions for single molecule measurement lead to high precision determination of a micro-amount of a biological material.

Abstract: In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.

Abstract: The object of the present invention is to acquire the brightness of NA>1 while alleviating the requirement for the precision of positioning for the collection lens of the sample cell in a non-liquid immersion system. In order to achieve the object mentioned above, the bottom of the sample cell is formed in a curved surface, and an arrangement is made to ensure that the fluorescence irradiated from the focusing point would be parallel pencils when emitted by the cell, and in addition a pinhole is disposed at the focal point of the fluorescence collection lens.

Abstract: An electrophoresis member is produced by laying a plurality of capillaries on an adhesive layer born on a support layer to form a capillary layer, laminating thereon a second support layer, and partially removing the first support layer, the first adhesive layer and the second support layer to partially expose the capillaries to form a window portion for irradiation and detection and a sample injection portion for injecting a sample.

Abstract: An electrophoresis member is produced by laying a plurality of capillaries on an adhesive layer born on a support layer to form a capillary layer, laminating thereon a second support layer, and partially removing the first support layer, the first adhesive layer and the second support layer to partially expose the capillaries to form a window portion for irradiation and detection and a sample injection portion for injecting a sample.

Abstract: Single molecule measurement is conducted by propagating a laser light under multiple reflection between two substrates constituting a flow channel for flowing a sample solution, exciting target molecules in the sample, detecting one-dimensional images of generated fluorescence by a one-dimensional detection portion, synthesizing two-dimensional images from the obtained one-dimensional images by a data synthesizing portion and measuring the concentration of target molecules in the sample solution by counting the number of target molecules based on the two-dimensional images, thereby measuring the concentration of the target molecules in the sample solution. These constitutions for single molecule measurement lead to high precision determination of a micro-amount of a biological material.

Abstract: An end detection type capillary electrophoresis apparatus that enables high-speed electrophoresis at a high resolution. The capillary electrophoresis apparatus has an inner diameter of at least 20 ?m and less than 80 ?m, and satisfies the constraint that the inner diameter/glass outer diameter?0.34. High fluorescence detection sensitivity is maintained and an analysis is carried out more quickly even as separation power improves.

Abstract: The disclosed invention provides methods and instruments for fluorescence detection making it possible to separate and detect analytes of a plurality of species in a migration (separation) channel with length of the order of millimeters. Analyte samples disperse across the whole detection region of a migration channel filled with a sieving matrix. Electrodes located in contact with a power supply and the sieving matrix cause the analytes to electophoretically migrate at predetermined velocity V. The detection region is irradiated by excitation light whose intensity changes in a cycle equaling pitch p in the direction that the analytes move. Fluorescence emission from the analytes exposed to the excitation light is detected by a detector. Fluctuation &dgr;i (t) of output current from the detector is analyzed by a spectrum analyzer and the obtained spectrum is displayed.

Abstract: An electrophoresis member is produced by laying a plurality of capillaries on an adhesive layer born on a support layer to form a capillary layer, laminating thereon a second support layer, and partially removing the first support layer, the first adhesive layer and the second support layer to partially expose the capillaries to form a window portion for irradiation and detection and a sample injection portion for injecting a sample.

Abstract: The disclosed invention provides methods and instruments for fluorescence detection making it possible to separate and detect analytes of a plurality of species in a migration (separation) channel with length of the order of millimeters. Analyte samples disperse across the whole detection region of a migration channel filled with a sieving matrix. Electrodes located in contact with a power supply and the sieving matrix cause the analytes to electophoretically migrate at predetermined velocity V. The detection region is irradiated by excitation light whose intensity changes in a cycle equaling pitch p in the direction that the analytes move. Fluorescence emission from the analytes exposed to the excitation light is detected by a detector. Fluctuation &dgr;i (t) of output current from the detector is analyzed by a spectrum analyzer and the obtained spectrum is displayed.

Abstract: According to an aspect of the present invention, there is provided an inspection apparatus using an optical interferometer including splitting and combining means (3) for splitting light from a light source (1) into incident light (41) irradiated on a sample and reference light (40) and combining signal light which is light scattered or reflected by the sample (7, 42) and the reference light, a modulator (4, 5, 10) for subjecting the reference light to phase modulation and a photo detector (9) for detecting light combined by the splitting and combining means (3), the inspection apparatus further including first detecting means (12-1) for detecting amplitudes of first signal components having frequencies of multiples of odd numbers of a fundamental modulation frequency of the modulator in a signal from the photo detector (9), second detecting means (12-2) for separating and detecting amplitudes of second signal components having frequencies of multiples of even numbers of the fundamental modulation frequency i