Abstract: It is an object of the present invention to provide a method for killing tumor cells, having a few side effects. The present invention provides a method for killing tumor cells, comprising: (1) a step of allowing an immunotoxin formed by binding an antibody binding to ROBO1 or a fragment thereof to a cytotoxin to come into contact with tumor cells; (2) a step of allowing a photosensitizer that induces photochemical cytoplasmic internalization to come into contact with the tumor cells; and (3) a step of irradiating the tumor cells with a wave length that is effective for activating the sensitizer, so as to kill the cells.

Abstract: A method, according to an embodiment, for evaluating a regenerated cartilage includes allowing a group of cells containing auricular chondrocytes to stand in the presence of a culture medium, subsequently collecting at least a portion of a liquid component from the culture medium, measuring the GFAP content of the collected liquid component, and determining whether a regenerated cartilage that has been obtained or can be obtained from the group of cells is suitable for transplantation based on the GFAP content.

Abstract: A method, according to an embodiment, for evaluating a regenerated cartilage includes allowing a group of cells containing auricular chondrocytes to stand in the presence of a culture medium, subsequently collecting at least a portion of a liquid component from the culture medium, measuring the GFAP content of the collected liquid component, and determining whether a regenerated cartilage that has been obtained or can be obtained from the group of cells is suitable for transplantation based on the GFAP content.

Abstract: A method for producing a bone filler basically comprising: a bone model producing step (step 1) for producing a bone model; a figure forming material filling step (step 2) for filling a figure forming material into a bone defect site of the bone model obtained in the bone model producing step; and a bone filler producing step (step 3) for producing a bone filler which is to be filled in a bone defect site based on the figure forming material filled in the bone defect site of the bone model in the figure forming material filling step.

Abstract: The object of the invention is to provide a redifferention medium and a redifferention method for making dedifferentiated chondrocyte to be redifferentiated into original chondrocyte, the dedifferentiated chondrocyte having attenuated cartilage characteristics due to dedifferentiation during in vitro culture. Accordingly, the invention provides a redifferention medium which is used for redifferentiating the dedifferentiated chondrocyte into the original chondrocyte, and contains insulin and at least one selected from BMP-2 and analogues thereof. The invention also provides a method for redifferentiating the dedifferentiated chondrocyte into the chondrocyte by culturing the dedifferentiated chondrocyte using the redifferentiation medium. The redifferentiation medium preferably further contains T3.