Abstract: The present disclosure relates to cytochrome P450 monooxygenases capable of oxidizing a monoterpenoid indole alkaloid (MIA) substrate and methods and uses thereof. The substrate may be a camptothecinoid, an evodiaminoid or an ellipticinoid. The disclosure further relates to method of producing hydroxylated monoterpenoid indole alkaloids, as well as derivatives and analogues of the produced hydroxylated monoterpenoid indole alkaloids. Pharmaceutical compositions comprising the hydroxylated monoterpenoid indole alkaloid derivatives are also provided.

Abstract: A vehicle includes an engine, a manual transmission having a clutch, and a controller. The controller is configured to, in response to receiving a signal indicative of a deceleration event initiated by the controller when the vehicle is operating with the clutch engaged, command the engine to shut down before an engine stall threshold to prevent a stall. A method of controlling a vehicle having an engine and a manual transmission with a clutch includes, in response to receiving a signal indicative of a deceleration event above a threshold value when the vehicle is operating with the clutch engaged, shutting down the engine before an engine stall threshold to prevent a stall.