Abstract: The present invention relates to processes for separating wheat flour into two or more fractions including a gluten fraction and a starch fraction, comprising the steps of: a) mixing wheat flour and water; b) adding one or more polypeptide (s) having GH8 xylanase activity; c) incubating the mixture for a predefined period of time; d) separating the mixture into two or more fractions including a gluten rich fraction and a starch rich fraction; and e) recovering the two or more fractions including a gluten rich fraction and a starch rich fraction.

Abstract: Provided are improved methods for treating wheat flour with lipase. Further provided are methods for separating wheat flour to provide a gluten fraction, a starch fraction and a fibre fraction where the wheat flour is treated with a selected lipase. Polypeptides having lipase activity, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides are also provided.