Abstract: A method for capturing ancient DNA of a wooden cultural relic, sequentially includes the following steps: sampling, removing exogenous DNA contamination on the inner part and the outer surface of a sample, and effectively obtaining the ancient DNA of the wooden cultural relic through vacuum freeze-drying dehydration, sample grinding, rinsing, cell lysis, DNA genome enrichment and hybridization and capturing. The method focuses on precise capture of the ancient DNA of the wood cells of the wood cultural relic, not only breaks through the problem that effective acquisition of the DNA of the wood cultural relic is difficult to realize by a conventional wood DNA extraction method, but also provides possibility for analysis and utilization of the ancient DNA with typical characteristics of serious exogenous contamination, low DNA content, high degradation, extensive damage and the like.

Abstract: A method for capturing ancient DNA of a wooden cultural relic, sequentially includes the following steps: sampling, removing exogenous DNA contamination on the inner part and the outer surface of a sample, and effectively obtaining the ancient DNA of the wooden cultural relic through vacuum freeze-drying dehydration, sample grinding, rinsing, cell lysis, DNA genome enrichment and hybridization and capturing. The method focuses on precise capture of the ancient DNA of the wood cells of the wood cultural relic, not only breaks through the problem that effective acquisition of the DNA of the wood cultural relic is difficult to realize by a conventional wood DNA extraction method, but also provides possibility for analysis and utilization of the ancient DNA with typical characteristics of serious exogenous contamination, low DNA content, high degradation, extensive damage and the like.