Abstract: Susceptibility of sessile microorganisms to antimicrobial agents is tested with a method comprising growing the microorganisms on a support to form a biofilm, contacting the biofilm with a metabolic substrate having a chromogenic and/or fluorogenic moiety to continue a base-line metabolic activity of the biofilm, contacting the biofilm with one or more antimicrobial agents, determining an experimental metabolic activity of the biofilm by measuring a signal from the metabolic substrate, and comparing the base-line metabolic activity with the experimental activity. The biofilm may be grown in a device comprising a plurality of wells, each well having a planar bottom and at least on wall, a plurality of supports comprising discs for growing the biofilm, each support disposed within a well perpendicular to the planar bottom, and at least one cover that fittably seals the top of each well.

Abstract: A cylinder lock plug that utilizes an axially moving sidebar. The sidebar is displaced axially by a key positioning mechanism on the blade of a uniquely configured key. Also on the key are unique short grooves on the lateral sides of the key blade running substantially perpendicular to the longitudinal axis of the key blade. The short grooves are configured complementary to an inner surface of the axially moving sidebar. Only the properly configured key can receive the sidebar as the key is turned to rotate the cylinder lock plug and the short grooves receive the inner surface. The invention is meant to be used with additional locking mechanisms, such as, for example, tumblers.