Abstract: The present invention provides lipid nanoparticle compositions, and methods of using the same, that are useful for introducing nucleic acids into eukaryotic cells, such as human immune cells. Generally, the lipid nanoparticles described herein comprise an apolipoprotein that is bound to one component of the lipid nanoparticles. The apolipoprotein can be bound to the lipid nanoparticle components by various types of bonds, including covalent bonds. The invention further provides methods for transfecting eukaryotic cells with such lipid nanoparticles, populations of eukaryotic cells, pharmaceutical compositions, and methods of treatment and use.

Abstract: The present disclosure provides methods for preparing a population of genetically-modified immune cells. The methods include contacting a population of immune cells with lipid nanoparticles in the presence of an apolipoprotein. The lipid nanoparticles include mRNA encoding an engineered nuclease having specificity for a recognition sequence in the genome of the immune cells. The mRNA is delivered into the immune cells and the engineered nuclease is expressed, generating a cleavage site at the recognition sequence. Further provided are populations of genetic ally-modified immune cells produced according to the disclosed methods, pharmaceutical compositions containing such cells, and methods of treating diseases with the genetically-modified immune cells.

Abstract: The subject matter described herein is directed to methods of modifying the micro-environment of a target cell or The methods comprise systemically administering to a subject a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap in the target cell, wherein the trap is expressed in the target cell thereby modifying the micro-environment. Also described herein are methods of reducing metastasis of a cancer comprising, systemically administering to a subject suffering from the cancer, a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap, wherein the trap is delivered to and then expressed in tissue susceptible to metastasis, wherein metastasis of the cancer to the tissue is reduced. Compositions for carrying out the methods are also described.

Abstract: The subject matter described herein is directed to methods of modifying the micro-environment of a target cell or The methods comprise systemically administering to a subject a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap in the target cell, wherein the trap is expressed in the target cell thereby modifiying the micro-environment. Also described herein are methods of reducing metastasis of a cancer comprising, systemically administering to a subject suffering from the cancer, a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap, wherein the trap is delivered to and then expressed in tissue susceptible to metastasis, wherein metastasis of the cancer to the tissue is reduced. Compositions for carrying out the methods are also described.

Abstract: Disclosed is a combined digital scorekeeper and vibration dampener device. The device is encased in a housing fabricated from a vibration dampening and/or shock absorption material. The device includes a plurality of string channels for attaching directly to the strings of a racket, and provides a miniature scoreboard that is operated by a simple mechanical or touch button system, so as not to distract the player's from the game. In an embodiment, the device is includes wireless technology to exchange scores and other data with other digital scorekeeper devices used by other players during a game, or via a network to a remote device to document the statistics from the match or provide data for other applications or sharing with others. In an embodiment, the digital scorekeeper includes one or more inertial sensors configured to detect and/or measure various data associated with the player's performance, which can be synced to video and used with training applications.

Abstract: The subject matter described herein is directed to methods of modifying the micro-environment of a target cell or The methods comprise systemically administering to a subject a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap in the target cell, wherein the trap is expressed in the target cell thereby modifiying the micro-environment. Also described herein are methods of reducing metastasis of a cancer comprising, systemically administering to a subject suffering from the cancer, a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap, wherein the trap is delivered to and then expressed in tissue susceptible to metastasis, wherein metastasis of the cancer to the tissue is reduced. Compositions for carrying out the methods are also described.

Abstract: Embodiments of the present invention relate to novel personal care compositions for cleaning and moisturizing the skin or hair comprising an extract of squash and/or pumpkin, a preservative and a cosmetic base. Other embodiments of the present invention relate to methods of making and methods of using the personal care compositions.

Abstract: Embodiments of the present invention relate to novel personal care compositions for cleaning and moisturizing the skin or hair comprising an extract of squash and/or pumpkin, a preservative and a cosmetic base. Other embodiments of the present invention relate to methods of making and methods of using the personal care compositions.