Abstract: Synthetic Notch receptors can be activated by ligands presented in different ways from a material, thereby serving as a general platform for generating new user-defined material-to-cell signaling pathways. Synthetic signals can be embedded directly into ECM proteins via genetic engineering of ECM-producing fibroblasts, on materials in 2D via microcontact printing or other micropatterning methods, and in 3D with immobilization such as covalent linking to hydrogels, in user-defined spatial patterns. Hence these material-to-cell pathways were used to spatially control activation of user-defined genetic programs in 2D and 3D. Since multiple synthetic pathways with different specificity can be engineered in a single cell, multicellular population can be patterned in up to three spatial domains of gene expression. We co-transdifferentiated fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns.

Abstract: A multilayer film having a heat seal layer and a high oxygen transmission layer. The high oxygen transmission layer being made from a blend of a heterogeneous ethylene/alpha-olefin copolymer; and at least one of: ethylene acrylate copolymer; homogeneous ethylene/alpha-olefin copolymer; ethylene acrylic acid; ethylene/vinyl acetate copolymer; and styrene butadiene block copolymer. The multilayer film having an oxygen transmission rate of at least 8,000 cc (STP)/m2/day/1 atm at 23 C at 0% RH measured according to ASTM 3985.

Abstract: A multilayer film having a heat seal layer and a high oxygen transmission layer. The high oxygen transmission layer being made from a blend of a heterogeneous ethylene/alpha-olefin copolymer; and at least one of: ethylene acrylate copolymer; homogeneous ethylene/alpha-olefin copolymer; ethylene acrylic acid; ethylene/vinyl acetate copolymer; and styrene butadiene block copolymer. The multilayer film having an oxygen transmission rate of at least 8,000 cc (STP)/m2/day/1 atm at 23C at 0% RH measured according to ASTM 3985.