Abstract: Painless, non-invasive nanofluidic delivery systems useful for the delivery of therapeutically active agents, GRAS agents, sterile fluids and inert fillers through epithelial membranes are described. The painless, non-invasive delivery system delivers a therapeutic agent via a micro-syringe-based assembly. The painless, non-invasive delivery system may also have a vehicle, which facilitates the absorption of the therapeutic agents by altering its absorption rate at the site of administration. Also disclosed is a method and components of the delivery system to administer therapeutic agents in a consistent, predictable and reproducible manner. Certain compositions may facilitate the delivery of different agents without altering the agents from their current or previous form.