Abstract: A surgical kit includes an applicator instrument and a needle assembly. The needle assembly provides two spaced pathways and carries a surgical fastener. One anchor of the surgical fastener is carried by one of the pathways and another anchor of the fastener is carried by the other pathway. A filament connects the two anchors of the fasteners and extends between the pathways. The applicator allows for simultaneous insertion of both anchors of the fastener at spaced locations with the filament extending from both anchors in the tissue and over the surface of the tissue or of a therapeutic implant fixated to the tissue. The needle assemblies may be removed from the applicator. The kit may include several needle assemblies that may be attached to the applicator in series to create a plurality of mattress-type stitches.