Abstract: A method of making a microplate, including: injection molding a resin to form a substrate (900) having a grating region feature on a surface of the substrate, and at least one micro-feature (910) in the vicinity of the grating region feature; waveguide treating (920) the resulting molded substrate; and over-molding the resulting waveguide treated molded substrate with a compatible resin to from the integral well plate (935) on the microplate. Also disclosed is a method of making a microplate, including: surface roughening to form a bonding area on a waveguide coated surface of a polymeric substrate having an integral grating region; and over-molding the resulting surface roughened substrate and a compatible resin to form the integral microplate, as defined herein.

Abstract: A resonant waveguide article, including: a polymeric substrate having at least one integral grating region, wherein the article has a low birefringence property of for example, from about 5 to 270 nm/cm, as defined herein. Also disclosed is a microplate including the resonant waveguide article, and an integral well plate bonded to the sensor article, as defined herein. Also disclosed are methods of making a sensor article, and a method of making and using the microplate including the sensor article, as defined herein.