Patents by Inventor Tyson Bowen

Tyson Bowen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for selectively amplifying and tagging nucleic acids
    Patent number: 10385387
    Abstract: Methods for adding sequence tags during amplification of a nucleic acid target are provided. The target is amplified using two sets of primers that are modified such that only tagged targets can ligate to hairpin adapters that protect them from subsequent exonuclease treatment. Methods of making circular nucleic acids are also provided. Specific overhangs are created by exonuclease trimming with a polymerase such that only desired targets can ligate to sticky ended hairpin adapters that protect them from subsequent exonuclease treatment. Compositions, kits, and systems related to or useful in the methods are also described.
    Type: Grant
    Filed: April 18, 2016
    Date of Patent: August 20, 2019
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Walter Lee, Tyson Bowen
  • METHODS FOR SELECTIVELY AMPLIFYING AND TAGGING NUCLEIC ACIDS
    Publication number: 20160304948
    Abstract: Methods for adding sequence tags during amplification of a nucleic acid target are provided. The target is amplified using two sets of primers that are modified such that only tagged targets can ligate to hairpin adapters that protect them from subsequent exonuclease treatment. Methods of making circular nucleic acids are also provided. Specific overhangs are created by exonuclease trimming with a polymerase such that only desired targets can ligate to sticky ended hairpin adapters that protect them from subsequent exonuclease treatment. Compositions, kits, and systems related to or useful in the methods are also described.
    Type: Application
    Filed: April 18, 2016
    Publication date: October 20, 2016
    Inventors: Walter Lee, Tyson Bowen