Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells for performing controlled serial elution techniques that enables execution of extraction/purification and splitting of analytes for multiplex detection via magnetic actuation only. Related magnetofluidic devices, kits, and methods are also provided.

Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells. Temperature sensitive materials are used to separate the sample inlet wells and sample analysis wells from one another prior to performing a given sample analysis application. Related magnetofluidic devices, kits, and methods are also provided.

Abstract: A tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet microfluidic chip; a valve control system connected to the tubing-free, sample-to-droplet microfluidic chip; a vacuum system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip; and a droplet formation pressure system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip. A microfluidic chip for a tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet interface section; a droplet mixing section in fluid connection with the tubing-free, sample-to-droplet interface section to received droplets therefrom; an incubation section in fluid connection with the droplet mixing section to receive droplets therefrom; and a detection section in fluid connection with the incubation section to receive droplets therefrom.

Abstract: Provided herein are microfluidic devices for analyzing samples. In one aspect, the microfluidic device includes a body structure having a droplet compression chamber, a sieve structure in fluid communication with the droplet compression chamber, which sieve structure comprises an array of protrusions that extend from at least one surface of the body structure and define at least a portion of one or more fluidic circuits, and a port at least partially disposed in the body structure. Other aspects include kits, methods, systems, computer readable media, and related aspects for analyzing samples.

Abstract: Methods for multiplexed detection of a nucleic acid sequence in a sample including the use of a plurality of oligonucleotide target-specific probes (TSPs) configured to bind to a distinct target nucleic acid sequence, where each of the TSPs includes one or more copies of a first fluorescent probe (FP) binding region and one or more copies of a second FP binding region, and where a predetermined ratio of the one or more copies of the first FP binding region to the one or more copies of the second FP binding region is indicative of the distinct target nucleic acid sequence the TSP is configured to bind to.

Abstract: Provided herein are methods of performing multiplexed real-time quantitative ratiometric regression PCR (qRR-PCR) to determine the fractional abundance of target variants in a population. Related systems and computer program products are also provided.

Abstract: Provided herein are methods of processing grain samples to detect the fractional abundance of transgenic traits. Related systems and computer program products are also provided.

Abstract: Described are methods for identifying antibiotic resistant bacteria, quantifying bacteria growth, and applying an antibiotic susceptibility test (AST) in one or more biological samples containing a bacteria and chips used in these methods.

Abstract: A method of separating, detecting and determining a size of each of a plurality of particles in a fluid includes compelling the fluid to flow through a fluid channel such that larger particles of the plurality of particles travel through the fluid channel faster than smaller particles of the plurality of particles; illuminating a detection zone of the fluid channel substantially uniformly across an entire cross section of the fluid channel such that each of the plurality of particles passes through illumination light upon passing through the detection zone; detecting each of the plurality of particles based on corresponding responses to the illuminating to determine a time that each of the plurality of particles passes through the detection zone; and determining a size of each of the plurality of particles based on the time that each of the plurality of particles passes through the detection zone.

Abstract: A method of separating, detecting and determining a size of each of a plurality of particles in a fluid includes compelling the fluid to flow through a fluid channel such that larger particles of the plurality of particles travel through the fluid channel faster than smaller particles of the plurality of particles; illuminating a detection zone of the fluid channel substantially uniformly across an entire cross section of the fluid channel such that each of the plurality of particles passes through illumination light upon passing through the detection zone; detecting each of the plurality of particles based on corresponding responses to the illuminating to determine a time that each of the plurality of particles passes through the detection zone; and determining a size of each of the plurality of particles based on the time that each of the plurality of particles passes through the detection zone.