Abstract: A ?-mannanase having increased enzymaic activity is disclosed. The ?-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.

Abstract: A ?-mannanase having increased enzymaic activity is disclosed. The ?-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.

Abstract: A xylanase having increased enzymaic activity is disclosed. The xylanase has a modified amino acid sequence of SEQ ID NO: 2, wherein Tryptophan at position 125 is substituted with Phenylalanine and Phenylalanine at position 163 is substituted with Tryptophan.

Abstract: One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3? end of the first segment connects to the 5? end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3? end of the third segment connects to the 5? end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.

Abstract: A glucanase having increased enzyme activity and thermostability is disclosed. The glucanase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein valine at position 18 is substituted with tyrosine and tryptophan at position 203 is substituted with tyrosine.

Abstract: A thermostable cellulase having increased enzyme activity is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the tyrosine at position 61 is substituted with an amino acid selected from the group consisting of phenylalanine, alanine and glycine.

Abstract: A real-time instruction device in accordance with the present invention comprises a database, a selecting module and a GUI. The database comprises at least multiple classifications and multiple Flash components corresponding to the classifications. The selecting interface allows a teacher to select a classification from the database. The GUI virtually simulates a white board that allows the teacher to dynamically drag a selected Flash component for real-time instruction with boundless space for teaching.

Abstract: A retaining ring for CMP is disclosed. The retaining ring has a plurality of grooves. The grooves have rounded sidewalls. Because the sidewalls of the grooves of the retaining ring are rounded, the slurry is not apt to accumulate around them and the pad is less scratched. Accordingly, the micro-scratches on the wafer surface are reduced and the yield of the CMP step is increased. Its operational method and application system are also disclosed in this invention.

Abstract: A method of removing an insulating layer on a substrate is described, including a first CMP process and a second CMP process performed in sequence, wherein the polishing slurry used in the first CMP process and that used in the second CMP process have substantially the same pH value that exceeds 7.0. A cleaning step is conducted between the first and the second CMP processes to remove a specific substance which would otherwise cause undesired particles to form in the second CMP process.