Abstract: Methods for the early sensing of tissue ischemia and/or infarctions within organs using the spectroscopic features of serum luminescence include (i) isolating serum from a blood sample, (ii) directing an excitation light at the serum, (iii) receiving an endogenous serum chromophore emission light from the excited serum, and (iv) determining a presence of an ischemic condition based on the intensity of the endogenous serum chromophore emission light. Methods of detecting organ dysfunction can include transmitting excitation light to luminal contents of a mammalian blood vessel comprising trace amounts of exogenous chromophores administered to the subject mammal via the circulatory system. Transdermal luminescence measurements from the trace amounts of exogenous chromophores can then be used to determine organ dysfunction.

Abstract: An image detection system for diagnosing a metabolic function and physiological status of a tissue or an organ having a fluorescent matter is provided. The image detection system includes a source emitting exciting light, a scan unit converting the exciting light into a scan of the exciting light, a light-guiding unit including an object lens, a splitter unit, and a detection unit. The splitter unit directs the scan of the exciting light to enter a tissue having a fluorescent matter such that the fluorescent matter in the tissue is excited by the scan of the exciting light and emits fluorescence light, whereby a layer of the tissue is scanned by the scan of the exciting light. The splitter unit directs the fluorescence light emitted from the tissue to the detection unit for processing so as to generate a fluorescent image of the layer of the tissue.

Abstract: The invention provides a method for the observation, identification, and detection of blood cells, which comprises a label-free third harmonic generation (THG) tomography having a property of least injury. Submicron morphologies and granularities of blood cells can be revealed and reflected through this method. Leukocytes with different granularities can thus be identified from the intensity and distribution of third harmonic generation signals generated within cells. Furthermore, the method of the present invention is capable of performing a noninvasive sectioning microscopy image in vivo. Without cell and tissue damage, label-free third harmonic generation microscopy can real-time observe the morphology and dynamics of blood cells flowing in vessels or trafficking in tissues; Red blood cells and leukocytes have different morphology in blood flow and can thus be distinguished by in vivo third harmonic generation microscopy.

Abstract: The present invention discloses microwave resonant absorption (MRA) of viruses through dipolar coupling to viral confined acoustic modes. The unique geometrical and mechanical properties of viruses can be reflected by the MRA frequencies, MRA linewidth, and the absorption selection rules of high-order acoustic modes. Combined these spectral characteristics with the mature microwave technology, this invention provides a novel physical mechanism to develop non-affinity-based rapid detection and identification of viruses. It can be applied in preliminary characterization on unknown or mutant viruses.

Abstract: Virus inactivation is performed with a specific microwave frequency to induce a collective vibration of virus through microwave resonant absorption (MRA).

Abstract: The present invention discloses microwave resonant absorption (MRA) of viruses through dipolar coupling to viral confined acoustic modes. The unique geometrical and mechanical properties of viruses can be reflected by the MRA frequencies, MRA linewidth, and the absorption selection rules of high-order acoustic modes. Combined these spectral characteristics with the mature microwave technology, this invention provides a novel physical mechanism to develop non-affinity-based rapid detection and identification of viruses. It can be applied in preliminary characterization on unknown or mutant viruses.

Abstract: A method for measuring an ultrashort optical pulse, in which third order autocorrelation of femtosecond (10−15 second) optical pulses was realized based on third-harmonic-generation (THG). A THG signal with three fundamental frequency photon contributed from three different split pulses of the ultrashort optical pulse is first generated. The three split pulses have time delays &tgr;1 and &tgr;2 in between. Then, the intensity of the THG signal is detected while varying the time delays &tgr;1 and &tgr;2 between the split pulses to obtain a triple correlation of the ultrashort optical pulse. The triple correlation and its Fourier transform are used to obtain the magnitude |Ĩ(&ngr;)| and the phase &agr;(&ngr;) of the ultrashort optical pulse intensity in the frequency domain, and the intensity of the ultrashort optical pulse in the time domain I(t) is determined using the magnitude |Ĩ(&ngr;)| and the phase &agr;(&ngr;) by inverse Fourier Transform.

Abstract: A method for measuring an ultrashort optical pulse, in which third order autocorrelation of femtosecond (10−15 second) optical pulses was realized based on third-harmonic-generation (THG). A THG signal with three fundamental frequency photon contributed from three different split pulses of the ultrashort optical pulse is first generated. The three split pulses have time delays &tgr;1 and &tgr;2 in between. Then, the intensity of the THG signal is detected while varying the time delays &tgr;1 and &tgr;2 between the split pulses to obtain a triple correlation of the ultrashort optical pulse. The triple correlation and its Fourier transform are used to obtain the magnitude |Ĩ(&ngr;)| and the phase &agr;(&ngr;) of the ultrashort optical pulse intensity in the frequency domain, and the intensity of the ultrashort optical pulse in the time domain I(t) is determined using the magnitude |Ĩ(&ngr;)| and the phase &agr;(&ngr;) by inverse Fourier Transform.