Abstract: An expandable microcapsule, which includes a core layer consisting of 300 parts by weight of foaming agent, 250 parts by weight of polymer water-absorbing material and 60 parts by weight of core layer lubricant, wherein the foaming agent has characteristics of reacting with water to generate gas; and a coating layer covering the surface of the core layer to form a granular structure, wherein the coating layer is consisting of 10% to 40% by weight of edible water-absorbent colloid, 5% to 40% by weight of functional health food additives, 2% to 30% by weight of emulsifier, 4% to 15% by weight of coating layer lubricant and 5% to 40% by weight of film coating composition, and wherein the film coating composition has characteristics of resistance to gastric acid decomposition and being decomposed only in the intestinal tract.

Abstract: A test method for a basic input/output system (BIOS), configured to test a computer device which includes the BIOS when a power on self test (POST) of the BIOS fails, is disclosed including following operations: enabling a fixing function of the BIOS by a debug port of a motherboard of the computer device; enabling a first memory device and disabling a second memory device, by the debug port; according to the fixing function, turning on the computer device by the first memory device; and determining whether the computer device is turned on successfully.

Abstract: A test method for a basic input/output system (BIOS), configured to test a computer device which includes the BIOS when a power on self test (POST) of the BIOS fails, is disclosed including following operations: enabling a fixing function of the BIOS by a debug port of a motherboard of the computer device; enabling a first memory device and disabling a second memory device, by the debug port; according to the fixing function, turning on the computer device by the first memory device; and determining whether the computer device is turned on successfully.

Abstract: A test method for testing a built-in memory in a computer device includes the following operations. The built-in memory is tested by a test function of a basic input/output system (BIOS) in the computer device to create a data file. An analysis application is performed by a test device to analyze the data file. According to analyzing the data file, an abnormal memory chip is determined whether to exist in the built-in memory. The data file includes test data of memory chips in the built-in memory.

Abstract: The present invention discloses a method of harnessing versatile phosphoramidation reactions to regioselectively link a group of alkynyl-/azido-containing compounds to post-synthetic nucleic acids primed with phosphate at the 5? termini. The method includes steps of: (a) monophosphoramidating H2N(CH2)nNH2 to the 5? end of the nucleic acid to form an intermediate 1 where n is an integer from 2 to 6, and the intermediate 1 has a formula of: (b) amidating the free —NH2 group of the intermediate 1 with to form an intermediate 2; and (c) reacting the terminal —N3 group of the intermediate 2 with 1-{3-{[4-(2-cyclooctyn-1-ylmethyl)benzoyl]amino}propyl}-4-{2-[4-(dimethylamino)phenyl]ethenyl}pyridinium hexafluorophosphate (Alkyne MegaStokes dye 608) by the SPAAC reaction to form modified nucleic acids. Without the presence of Cu(I), the modified nucleic acids were subjected to azide-alkyne cycloaddition in an aqueous solution to obtain various nucleic acid conjugates with a high yield.

Abstract: The present invention discloses a method of harnessing versatile phosphoramidation reactions to regioselectively incorporate alkynyl/azido groups into post-synthetic nucleic acids primed with phosphate at the 5? termini. With and without the presence of copper, the modified nucleic acids were subjected to azide-alkyne cycloaddition to obtain various nucleic acid conjugates including a peptide-oligonucleotide conjugate (POC) with a high yield.

Abstract: Methods are provided to label nucleic acids through two-step phosphoramidation reactions with regiospecificity and high sensitivity. The methods can be widely used for synthesizing conjugates of nucleic acids. Through the two-step phosphoramidation reactions, nucleic acids are directly or indirectly labeled for easier and more efficient applications. The present invention also uses fluorescence quenching and DNA melting profile studies to demonstrate that the nucleic acids modified through the two-step phosphoramidation reactions preserve intrinsic base-pairing specificity and bestow regioselectivity in nucleic acids with high yield. Thus, the present invention has significant contribution to basic nucleic acid research; and, in the future, can be applied to effectively and quantitatively detect and measure nucleic acids inside and outside living organisms.

Abstract: A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5?-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.

Abstract: A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5?-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.

Abstract: A clutch consists of transmission gears, pistons, a switch on each piston, and a passive gear; the transmission gears are fitted on a fly wheel; each piston is pivoted to an inner side of a clutch cover at a fixed end, and pivoted to a respective transmission gear at a moving end; the switches are provided for changing valves of the pistons between closed and open states, and have resilience so that they will return to closed position automatically when external force moving them to open position disappears; the passive gear is joined to a clutch shaft and engaged with the transmission gears; when the piston valves are in closed state, the piston moving ends will be immobilized to fix the transmission gears; therefore, the transmission gears will cause rotation of the passive gear and the clutch shaft when the fly wheel is rotating.