Abstract: Provided is a label-free, single biological cell dielectric spectroscopy method, including the steps of: translocating a biological cell through a micropore or channel embedded in a substrate and interfaced with a coplanar waveguide while the biological cell experiences at least one RF field of at least 700 MHz provided via an RF input port to the coplanar waveguide; performing a time domain measurement of at least one RF signal reflected from or transmitted to a device under test (DUT); and determining an amplitude change and a phase change based on the reflected or transmitted at least one RF signal due to the translocating biological cell to determine an internal state or a morphological state of the biological cell. Also disclosed herein are devices for performing the dielectric spectroscopy method.