Abstract: Methods for increasing the genetic stability of genetically enhanced microbial host cells capable of producing a compound of interest are disclosed.

Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyanoI, is disclosed. The enhanced Cyanobacterium sp. ABICyanoI produces a compound or compounds of interest.

Abstract: Methods for increasing the genetic stability of genetically enhanced microbial host cells capable of producing a compound of interest are disclosed.

Abstract: This invention provides a method for the prolonged production of ethanol using a metabolically enhanced cyanobacterium.

Abstract: One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: This invention provides a method for the prolonged production of ethanol using a metabolically enhanced cyanobacterium.

Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

Abstract: This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds.

Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyanoI, is disclosed. The enhanced Cyanobacterium sp. ABICyanoI produces a compound or compounds of interest.

Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: A cyanobacterium for the production of isoprene having an extrachromosomal plasmid harboring a gene for the production of isoprene. Such a cyanobacterium exhibits a higher isoprene production rate than other conventional strains.

Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

Abstract: One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Abstract: This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds.