Abstract: A method is used for the qualitative evaluation of real-time PCR data, where a time/PCR amplification plot of an associated sample is classified as a negative plot or as a positive plot. The method involves providing a real-time PCR amplification plot to be classified, plotting at least 20 successive amplitude values of corresponding successive PCR cycle indices of the sample. Next, a quality metric is determined, on the basis of the at least one amplitude value. A first criterion is determined by a comparison of the quality metric with a first standard value. A sequence of values is then determined, which indicates a gradient of the PCR amplification plot to be classified, and a second criterion is determined as to whether the sequence of values exceeds a second standard value. Finally, the real-time PCR amplification plot is classified as a positive plot if all the criteria given above are satisfied.

Abstract: A nucleic acid or peptide detection system that includes a carrier with at least two areas is provided. A method and kit that use and/or include the nucleic acid peptide detection system are also provided.

Abstract: A method is useful for automated detection of antibodies in a liquid biological sample with an antigen chip having antigen spots which are applied thereon and which have an identical, common dye. The antigen spots form respective antigen spot sets which form corresponding respective, regular antigen spot patterns. Furthermore, reference spots comprising the identical dye are applied, and form a reference spot set which forms a regular reference pattern. The reference pattern differs with respect to its regularity from the antigen spot patterns. Through a first image information item which represents a color of the reference spots and of the antigen spots due to the identical, first dye and through a second image information item which represents a potential color of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by image processing.

Abstract: A method is useful for automated detection of antibodies in a liquid biological sample with an antigen chip having antigen spots which are applied thereon and which have an identical, common dye. The antigen spots form respective antigen spot sets which form corresponding respective, regular antigen spot patterns. Furthermore, reference spots comprising the identical dye are applied, and form a reference spot set which forms a regular reference pattern. The reference pattern differs with respect to its regularity from the antigen spot patterns. Through a first image information item which represents a color of the reference spots and of the antigen spots due to the identical, first dye and through a second image information item which represents a potential color of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by image processing.

Abstract: An antigen chip is described, the latter comprising a plane substrate surface; and antigen spots, which are applied in a predetermined pattern on the substrate surface and which contain a first fluorescence dye. Further, an apparatus and a method for detecting antibodies in a sample are provided. Even further, the application relates to kit for use in a method for detecting antibodies in a sample.