Patents by Inventor Ulfert Deneke
Ulfert Deneke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6214570Abstract: The subject matter of the application is a method for the separation of non-HDL lipoproteins from biological fluids in a carrier through which liquids can flow. The carrier contains a precipitating agent for non-high density lipoproteins (non-HDLs).Type: GrantFiled: July 14, 1998Date of Patent: April 10, 2001Assignee: Roche Diagnostics GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert
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Patent number: 6171849Abstract: An apparatus comprising a first porous carrier and a second porous carrier for evaluating biological fluid samples is disclosed. The apparatus is used for separating non high density lipoprotein (non-HDL) from a lipoprotein in a body sample and for determining high density lipoprotein (HDL) cholesterol in a HDL and non high density lipoprotein (non-HDL) in a body sample.Type: GrantFiled: January 12, 2000Date of Patent: January 9, 2001Assignee: Roche Diagnostics GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert
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Patent number: 5786164Abstract: The invention relates to a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDL-s are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.Type: GrantFiled: June 11, 1996Date of Patent: July 28, 1998Assignee: Boehringer Mannheim GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert
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Patent number: 5580743Abstract: The invention relates to a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.Type: GrantFiled: February 6, 1995Date of Patent: December 3, 1996Assignee: Boehringer Mannheim GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert
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Patent number: 5426030Abstract: The invention relates to an apparatus and a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.Type: GrantFiled: August 24, 1990Date of Patent: June 20, 1995Assignee: Boehringer Mannheim GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert
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Patent number: 5374546Abstract: The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.Type: GrantFiled: January 16, 1992Date of Patent: December 20, 1994Assignee: Boehringer Mannheim GmbHInventors: Rolf Nagel, Ulfert Deneke, Jurgen Mistele
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Patent number: 5274095Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and /or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are useful as redox indicators, particularly in the presence of a SH group-containing substance.Type: GrantFiled: August 11, 1992Date of Patent: December 28, 1993Assignee: Boehringer Mannheim GmbHInventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
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Patent number: 5162200Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and/or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are used as redox indicators in the colorimetric diarylimidazoles are not influenced by the presence of a SH group-containing substance. The peroxidate-acting substance can be used to determine the presence of creatine kinase.Type: GrantFiled: May 30, 1990Date of Patent: November 10, 1992Assignee: Boehringer Manneheim GmbHInventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
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Patent number: 5108913Abstract: .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.Type: GrantFiled: June 27, 1990Date of Patent: April 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Elli Rauscher, Ulrich Neumann, August W. Wahlefeld, Alexander Hagen, Wolfgang Gruber, Joachim Ziegenhorn, Eugen Schaich, Ulfert Deneke, Michal Gerhard, Gunter Weimann
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Patent number: 4966855Abstract: The invention provides new redox indicators and uses for these redox compounds (I): ##STR1## Wherein X is oxygen or sulphur, R.sub.1 is julolidine or tetrahydroquinoline, which can carry an alkyl radical on the nitrogen atom which, in turn, can be substituted by a sulphuric, phosphonic or carboxylic acid residues, or is (II); wherein R.sub.4 is hydroxyl or a mono- or dialkylated amino group, R.sub.5 and R.sub.6, which can be the same or different, are hydrogen, alkyl or alkoxy, R.sub.2 is hydrogen or alkyl, julolidine or tetrahydroquinoline, which can carry an alkyl on the nitrogen atom which alkyl can be substituted by sulphuric, phosphonic or carboxylic acids, or is III; wherein R.sub.5 and R.sub.6 are the same as in R.sub.1 and R'.sub.4 is hydroxyl, an amino or a mono-or dialkylated amino group, whereby the alkyl radicals can be substituted one or more times by hydroxyl, alkoxy, halogen, morpholine or a sulphuric, carboxylic or phosphonic acid residue, which acids can also be esterified, and R.sub.Type: GrantFiled: July 2, 1985Date of Patent: October 30, 1990Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Werner Guthlein, Wolfgang Weckerle, Hans Wielinger
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Patent number: 4923796Abstract: In a method for the quantative enzymatic determination of adenosine diphosphate (ADP) in aqueous solution by phosphorylation of the ADP with a kinase in the presence of a phosphorylated substrate therefor, with the formation of ATP and a dephosphorylated substrate, such as galactose, formate, pyruvate or nucleoside diphosphate, the dephosphorylated substrate is reacted, optionally with the insertion of a further adjuvant reaction, such as a decarboxylation, transamination or hexosidiation, with NAD(P) in the presence of a dehydrogenase and the NAD(P)H formed is either measured directly or reacted with a tetrazolium salt in the presence of an electron carrier, with the formation of a formazane, which latter is then measured.Type: GrantFiled: January 17, 1989Date of Patent: May 8, 1990Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Gerhard Micharl, Hans-Otto Beutler
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Patent number: 4876067Abstract: The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent layeType: GrantFiled: August 25, 1987Date of Patent: October 24, 1989Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Rolf Nagel, Anselm Rothe, Helmut Freitag
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Patent number: 4665023Abstract: The present invention provides imidazole derivatives of the general formula: ##STR1## wherein R, R.sub.1 and R.sub.2 are defined hereinbelow. The present invention is also concerned with processes for the preparation of these imidazole derivatives and with the use thereof as redox indicators.Type: GrantFiled: March 29, 1985Date of Patent: May 12, 1987Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Werner Guthlein, Manfred Kuhr, Hartmut Merdes, Hans-Rudiger Murawski, Hans Wielinger
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Patent number: 4603107Abstract: The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity.The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:.gamma.-GT-arylamide:1-100 mmol/l..gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l.coupling component:1-500 mmol/l.oxidation agent:0.1-500 mmol/l.buffer:10-1000 mmol/l.Type: GrantFiled: September 16, 1983Date of Patent: July 29, 1986Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Rolf Nagel, Walter Rittersdorf
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Patent number: 4591553Abstract: The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxypheny l]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.Type: GrantFiled: June 1, 1983Date of Patent: May 27, 1986Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Wolfgang Gruber, Hans-Georg Batz
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Patent number: 4563422Abstract: The present invention provides a process for removing ascorbic acid from aqueous solutions by the addition of ascorbate oxidase, wherein catalase and hydrogen peroxide are also added to the solution.The present invention also provides an agent for the removal of ascorbic acid from aqueous solutions, based on a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide and optionally also a stabilizer and/or buffer substance.Type: GrantFiled: May 21, 1982Date of Patent: January 7, 1986Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Hans Lange, Walter Rittersdorf
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Patent number: 4544631Abstract: .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.Type: GrantFiled: October 16, 1981Date of Patent: October 1, 1985Assignee: Boehringer Mannheim GmbHInventors: Elli Rauscher, Ulrich Neumann, August W. Wahlefeld, Alexander Hagen, Wolfgang Gruber, Joachim Ziegenhorn, Eugen Schaich, Ulfert Deneke, Gerhard Michal, Gunter Weimann
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Patent number: 4271265Abstract: In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of .alpha.-ketoglutarate in a buffered solution, the .alpha.-ketoglutarate formed is reacted with .gamma.-aminobutyrate in the presence of .gamma.-aminobutyrate transaminase with formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate semialdehyde dehydrogenase to give NADPH, and the latter is measured either directly or after conversion with a tetrazolium salt and an electron carrier such as diaphorase, phenantroline methosulphate or phenazine methosulphate to a formazane dye.A reagent suited for use with this method contains .gamma.-aminobutyrate transaminase, succinate semialdehyde dehydrogenase, .gamma.-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.Type: GrantFiled: July 20, 1979Date of Patent: June 2, 1981Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Peter Stahl, Walter Schneider
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Patent number: 4168205Abstract: In a process for the determination of substrates or enzyme activities in a test batch, utilizing a conventional redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase; the addition of ascorbate oxidase substantially eliminates the interfering effect of ascorbic acid frequently found in the test sample.Type: GrantFiled: April 28, 1977Date of Patent: September 18, 1979Assignee: Boehringer Mannheim GmbHInventors: Josef Danninger, Ulfert Deneke, Gunter Lang, Gerhard Michal, Peter Roeschlau
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Patent number: RE39915Abstract: An apparatus comprising a first porous carrier and a second porous carrier for evaluating biological fluid samples is disclosed. The apparatus is used for separating non high density lipoprotein (non-HDL) from a lipoprotein in a body sample and for determining high density lipoprotein (HDL) cholesterol in a HDL and non high density lipoprotein (non-HDL) in a body sample.Type: GrantFiled: August 20, 2003Date of Patent: November 6, 2007Assignee: Roche Diagnostics GmbHInventors: Walter Rittersdorf, Ulfert Deneke, Gerhard Hiller, Hartmut Merdes, Klaus Buecker, Uwe Goebbert