Abstract: A process for generating sequence-specific proteases by screening-based directed evolution is disclosed. The use of the process provides proteases recognizing and cleaving user-definable amino-acid sequences with high sequence-specificity. Proteases obtainable by the process can be used in a variety of medical, diagnostic and industrial applications.

Abstract: The present invention provides an insertion, deletion and/or substitution mutein of wild-type Trichoderma reesei ?-mannanase having enhanced thermostability, proteolytic stability, specific activity and/or stability at low pH, a nucleic acid molecule encoding said mannanase mutein, a composition comprising said mannanase mutein; a method for its preparation, and its use for food and feed processing, for coffee extraction and the processing of coffee waste, as a supplement to food and feed, for enzyme aided bleaching of paper pulps, as bleaching and/or desizing agent in textile industry, for oil and gas well stimulation by hydraulic fracturing, as detergent, for removal of biofilms and in delivery systems, or for the processing of renewable resources intended for the production of biological fuels.

Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.

Abstract: The present invention provides variants of serine proteases of the S1 class with altered sensitivity to one or more activity-modulating substances. A method for the generation of such proteases is disclosed, comprising the provision of a protease library encoding polynucleotide sequences, expression of the enzymes, screening of the library in the presence of one or several activity-modulating substances, selection of variants with altered sensitivity to one or several activity-modulating substances and isolation of those polynucleotide sequences that encode for the selected variants.

Abstract: The present invention provides method for the treatment of a disease by applying a medicament comprising a protease with a defined specificity is capable to hydrolyze specific peptide bonds within a target substrate related to such disease. The proteases with such a defined specificity can further be used for related therapeutic or diagnostic purposes.

Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.

Abstract: The present invention provides method for the treatment of a disease by applying a medicament comprising a protease with a defined specificity is capable to hydrolyze specific peptide bonds within a target substrate related to such disease. The proteases with such a defined specificity can further be used for related therapeutic or diagnostic purposes.

Abstract: A process for generating sequence-specific proteases by screening-based directed evolution is disclosed. The use of the process provides proteases recognizing and cleaving user-definable amino-acid sequences with high sequence-specificity. Proteases obtainable by the process can be used in a variety of medical, diagnostic and industrial applications.

Abstract: The present invention provides subtilisin variants with improved characteristics, including improved substrate affinity, catalytic activity, catalytic efficiency and stability under washing conditions as well as overall wash performance. The subtilisin variants are therefore useful additives in cleaning compositions.

Abstract: The invention relates to a device for the continuous production of plate-shaped products, comprising two endless strips which are positioned one above the other and can be driven. A working gap is formed between the sides of said endless strips, said sides being adjacent to one another and each being conveyed via a support surface. The starting material for processing is conveyed through said working gap which is bordered on each side by an accompanying strip. Said accompanying strip is situated along said working gap between the two endless strips and moves with them, consisting of a flexible sealing strip which has a friction surface on at least one face, said face facing towards an endless strip. Said friction surface increases the static friction between the flexible sealing strip and the endless strip.

Abstract: A continuously operating press is described for the manufacture and/or coating of board webs consisting of a fleece or mat in which the press belts are supported on a lubricant film formed on a slide lining. For the marginal width adjustment of the press zone the slide linings are subdivided into a main zone and adjoining marginal strip zones respectively. In one embodiment of the invention transition zones are in each case provided between two of these slide lining zones and are either made groove-free or provided with blind grooves, which serve neither as supply nor as discharge for lubricant. In a further embodiment of the invention adjacent grooves in these slide lining zones are alternatively provided with supply and discharge openings for the lubricant.

Abstract: A lubrication system for a continuously operating press for the pressing of a multi-ply board web (119) of pre-manufactured material has a press gap defined between upper and lower runs of endless recirculating steel press bands (105). A slide coating (101) which extends over the width of the press zone is provided with grooves. Each press band (105) slides by means of a lubricant which is supplied into the slide coating via supply lines and is led away through discharge openings. Several grooves (107, 108, 110, 111) for the swirling of the lubricant are provided for the press band (105) at least in the two outer longitudinal edge regions of the slide coating.