Patents by Inventor Ulrich Zähringer
Ulrich Zähringer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11360085Abstract: Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-flee aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the Limulus amoebocyte lysate assay.Type: GrantFiled: January 28, 2020Date of Patent: June 14, 2022Assignees: Forschungszentrum Borstel Leibniz Lungenzentrum, Hoffmann-La Roche Inc.Inventors: Christian Alexander, Sven Deutschmann, Pierre Lang, Friedrich Von Wintzingerode, Ulrich Zaehringer
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Publication number: 20200240988Abstract: Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-flee aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate assay.Type: ApplicationFiled: January 28, 2020Publication date: July 30, 2020Inventors: Christian ALEXANDER, Sven DEUTSCHMANN, Pierre LANG, Friedrich VON WINTZINGERODE, Ulrich ZAEHRINGER
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Patent number: 10585097Abstract: Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-free aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate assay.Type: GrantFiled: July 27, 2016Date of Patent: March 10, 2020Assignees: Forschungszentrum Borstel Leibniz Lungerzentrum, Hoffmann-La Roche Inc.Inventors: Christian Alexander, Sven Deutschmann, Pierre Lang, Friedrich Von Wintzingerode, Ulrich Zaehringer
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Publication number: 20180188249Abstract: Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-free aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate as say.Type: ApplicationFiled: July 27, 2016Publication date: July 5, 2018Inventors: Christian ALEXANDER, Sven DEUTSCHMANN, Pierre LANG, Friedrich VON WINTZINGERODE, Ulrich ZAEHRINGER
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Patent number: 9611441Abstract: The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic microorganism, with an increased content of fatty acids, oils or lipids with ?6 double bonds owing to the expression of a moss ?6-desaturase. The invention furthermore relates to transgenic organisms comprising a ?6-desaturase gene, and to the use of unsaturated fatty acids ort of the triglycerides with an increased content if unsaturated fatty acids prepared in the process.Type: GrantFiled: May 2, 2014Date of Patent: April 4, 2017Assignee: BASF Plant Science GmbHInventors: Ernst Heinz, Thomas Girke, Jodi Scheffler, Oswaldo Da Costa e Silva, Hermann Schmidt, Ulrich Zähringer, Ralf Reski
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Publication number: 20170066994Abstract: The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic microorganism, with an increased content of fatty acids, oils or lipids with ?6 double bonds owing to the expression of a moss ?6-desaturase. The invention furthermore relates to transgenic organisms comprising a ?6-desaturase gene, and to the use of unsaturated fatty acids ort of the triglycerides with an increased content if unsaturated fatty acids prepared in the process.Type: ApplicationFiled: May 2, 2014Publication date: March 9, 2017Applicant: BASF Plant Science GmbHInventors: Ernst Heinz, Thomas Girke, Jodi Scheffler, Oswaldo Da Costa e Silva, Hermann Schmidt, Ulrich Zähringer, Ralf Reski
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Patent number: 8933300Abstract: The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Type: GrantFiled: March 5, 2007Date of Patent: January 13, 2015Assignee: BASF AktiengesellschaftInventors: Ernst Heinz, Thorsten Zank, Ulrich Zähringer, Jens Lerchl, Andreas Renz
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Patent number: 8835715Abstract: The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an increased content of fatty acids, oils or lipids with ?6 double bonds owing to the expression of a moss ?-6-desaturase [sic]. The invention furthermore relates to transgenic organisms comprising a ?6-desaturase gene, and to the use of the unsaturated fatty acids or of the triglycerides with an increased content of unsaturated fatty acids prepared in the process.Type: GrantFiled: July 4, 2000Date of Patent: September 16, 2014Assignee: BASF Plant Science GmbHInventors: Ernst Heinz, Thomas Girke, Jodi Scheffler, Oswaldo Da Costa E Silva, Hermann Schmidt, Ulrich Zähringer, Ralf Reski
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Publication number: 20140235733Abstract: The present invention relates to an improved process for the preparation of unsaturated fatty acids and to a process for the preparation of triglycerides with an increased content of unsaturated fatty acids. The invention relates to the generation of transgenic microorganism, with an increased content of fatty acids, oils or lipids with ?6 double bonds owing to the expression of a moss ?6-desaturase. The invention furthermore relates to transgenic organisms comprising a ?6-desaturase gene, and to the use of unsaturated fatty acids ort of the triglycerides with an increased content if unsaturated fatty acids prepared in the process.Type: ApplicationFiled: May 2, 2014Publication date: August 21, 2014Applicant: BASF Plant Science GmbHInventors: Ernst Heinz, Thomas Girke, Jodi Scheffler, Oswaldo Da Costa e Silva, Hermann Schmidt, Ulrich Zähringer, Ralf Reski
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Patent number: 8729337Abstract: The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.Type: GrantFiled: January 2, 2012Date of Patent: May 20, 2014Assignee: BASF Plant Science GmbHInventors: Jens Lerchl, Andreas Renz, Ernst Heinz, Frederic Domergue, Ulrich Zähringer
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Publication number: 20120210465Abstract: The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.Type: ApplicationFiled: January 2, 2012Publication date: August 16, 2012Applicant: BASF Plant Science GmbHInventors: Jens Lerchl, Andreas Renz, Ernst Heinz, Frederic Domergue, Ulrich Zähringer
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Patent number: 8088974Abstract: The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11 encoding polypeptides having desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.Type: GrantFiled: September 24, 2009Date of Patent: January 3, 2012Assignee: BASF Plant Science GmbHInventors: Jens Lerchl, Andreas Renz, Ernst Heinz, Frederic Domergue, Ulrich Zähringer
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Patent number: 7714185Abstract: The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis.Type: GrantFiled: December 11, 2003Date of Patent: May 11, 2010Assignee: University of BristolInventors: Jonathan A. Napier, Olga Sayanova, Colin M. Lazarus, Baoxiu Qi, Ernst Heinz, Thorsten Zank, Ulrich Zähringer
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Patent number: 7629503Abstract: The present invention relates to an improved process for the specific preparation of unsaturated ?-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of ?-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having ?-4 double bonds owing to the expression of a ?-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof.Type: GrantFiled: April 6, 2004Date of Patent: December 8, 2009Assignee: BASF Plant Science GmbHInventors: Petra Cirpus, Jörg Bauer, Astrid Meyer, Ernst Heinz, Ulrich Zähringer
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Patent number: 7615679Abstract: The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention further relates to the use of the nucleic acid sequence of SEQ ID NO: 1 encoding a polypeptide having delta-5 desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or microorganism with an increased content of fatty acids, oil or lipids with unsaturated long chain fatty acids, to their homologs or derivatives, and to gene constructs encompassing these genes. The invention also relates to the use of the nucleic acid sequences alone or in combination with further desaturases and/or elongase genes in the method and for generating transgenic organisms.Type: GrantFiled: January 18, 2002Date of Patent: November 10, 2009Assignee: BASF Plant Science GmbHInventors: Jens Lerchl, Andreas Renz, Ernst Heinz, Frederic Domergue, Ulrich Zähringer
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Patent number: 7544859Abstract: The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Type: GrantFiled: February 8, 2001Date of Patent: June 9, 2009Assignee: BASF AktiengesellschaftInventors: Ernst Heinz, Thorsten Zank, Ulrich Zähringer, Jens Lerchl, Andreas Renz
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Patent number: 7183458Abstract: The present invention relates to a process for preparing unsaturated fatty acids and to a process for preparing triglycerides with an increased content of unsaturated fatty acids. The invention further relates to the use of DNA sequences coding for ?6-acetylenase/?6-desaturases or ?6-desaturases for producing a transgenic organism, preferably a transgenic plant or a transgenic microorganism with increased content of fatty acids, oils or lipids with ?6 triple bonds and/or ?6 double bonds. The invention additionally relates to an isolated nucleic acid sequence; to an expression cassette comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or expression cassette. The invention additionally relates to unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and the use thereof.Type: GrantFiled: June 7, 2000Date of Patent: February 27, 2007Assignee: BASF Plant Science GmbHInventors: Ernst Heinz, Sten Stymne, Michael Lee, Thomas Girke, Petra Sperling, Ulrich Zaehringer
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Publication number: 20060218668Abstract: The present invention relates to an improved process for the specific preparation of unsaturated ?-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of ?-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having ?-4 double bonds owing to the expression of a ?-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof.Type: ApplicationFiled: April 6, 2004Publication date: September 28, 2006Applicant: BASF Plant Science GmbHInventors: Petra Cirpus, Jörg Bauer, Astrid Meyer, Ernst Heinz, Ulrich Zähringer
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Patent number: 6833451Abstract: Lipopolysaccharides and processes for producting the lipopolysaccharides are provided. The lipopolysaccharide has a lipid A portion, a core oligosaccharide portion, and an O-specific chain having a single repeating unit 06. A lipopolysaccharide may be produced by washing and drying and E. coli bacterial mass, subjecting the washed and dried bacterial mass to a phenol/water extraction, and treating the extract with RNases, DNases, and proteinase K.Type: GrantFiled: December 23, 2002Date of Patent: December 21, 2004Assignee: Pharma-Zentrale GmbHInventors: Hans Proppert, Jürgen Malinka, Jürgen Schulze, Ulrich Sonnenborn, Ulrich Zähringer, Artur Ulmer, Ernst Theodor Rietschel
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Patent number: 6743606Abstract: The invention relates to a protein which presents identical or different catalytically active domains of glycosyltransferases and has a processive action. In particular, the same protein is successively active in at least two successive process steps.Type: GrantFiled: September 22, 2000Date of Patent: June 1, 2004Assignees: Gesellschaft Für Erwerb und Verwertung von Schutzrechten-GVS mbH, Forschungszentrum BorstelInventors: Frank P. Wolter, Petra Jorasch, Ernst Heinz, Ulrich Zähringer