Patents by Inventor Uma Mahesh Babu
Uma Mahesh Babu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9372192Abstract: A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device.Type: GrantFiled: February 8, 2013Date of Patent: June 21, 2016Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine
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Publication number: 20160084832Abstract: A lateral flow assay is capable of detecting and differentiating viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In some preferred embodiments, bimodal methods and devices determine if an infection is bacterial and/or viral. A dual use two strip sample analysis device includes a first lateral flow chromatographic test strip to detect MxA and a low level of C-reactive protein and a second lateral flow chromatographic test strip to detect high levels of C-reactive protein. In some preferred embodiments, the sample is a fingerstick blood sample.Type: ApplicationFiled: December 2, 2015Publication date: March 24, 2016Inventors: Robert P. Sambursky, Robert W. VanDine, Uma Mahesh Babu, Peter Condon
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Patent number: 9250236Abstract: The present invention includes methods and devices for preventing interfering substances from affecting the accuracy of a lateral flow immunoassay. In preferred embodiments, a test strip includes a capturing zone that includes at least one mobile capturing reagent that separates at least one interfering substance from the analyte. The capturing zone is preferably located upstream of the sample application zone. In some embodiments, the reagent/conjugate zone is also located upstream of the sample application zone. The capturing zone may be located upstream, downstream, or overlapping with the reagent/conjugate zone in these embodiments. In other preferred embodiments, one or more mobile capturing reagents are included in the elution medium/running buffer. In yet other embodiments, the capturing reagent is incorporated into a sample collection device of a sample collection system, preferably separate from the chromatographic test strip. A lysis zone is also included in some preferred embodiments.Type: GrantFiled: March 13, 2013Date of Patent: February 2, 2016Assignee: Rapid Pathogen Screening, Inc.Inventors: Uma Mahesh Babu, Franz Aberl, Marcus Scheibenzuber, Robert P. Sambursky, Robert W. VanDine, Jose S. Sambursky
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Patent number: 9212386Abstract: Methods and devices for detecting a target enzyme include an anchored or trapped peptide complex. The peptide complex includes an anchor particle immobilized on a sample analysis device or trapped in a reaction receptacle including a filter, a peptide, with at least one enzyme cleavage site for a target enzyme, bound to the anchor particle, at least one detectable label, and at least one first tag bound to the peptide on a side of the enzyme cleavage site opposite the anchor particle. When the target enzyme is present in the sample, the enzyme cleaves the peptide at the enzyme cleavage site, permitting the cleaved peptide to reach the test zone of a sample analysis device such that the first tag binds to the immobilized second tag and a signal is detected at the test zone.Type: GrantFiled: July 19, 2012Date of Patent: December 15, 2015Assignee: Rapid Pathogen Screening, Inc.Inventors: Uma Mahesh Babu, Robert P. Sambursky, Robert W. VanDine
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Publication number: 20150323527Abstract: Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer.Type: ApplicationFiled: July 22, 2015Publication date: November 12, 2015Inventors: Uma Mahesh Babu, Robert P. Sambursky
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Publication number: 20150273026Abstract: Full length MxA constructs and truncated MxA constructs produce human MxA protein in E. coli. The full length MxA and truncated MxA constructs are preferably E. coli codon-optimized to optimize the amount of protein made using the constructs. T5 or T7 promoters can each be used in combination with either the full length MxA or the truncated MxA constructs. In one preferred embodiment, the MxA protein produced by the full length MxA or truncated MxA constructs is used in a control prep or external control. In other preferred embodiments, the MxA protein is used as a therapeutic.Type: ApplicationFiled: June 17, 2015Publication date: October 1, 2015Inventors: Robert W. VanDine, Robert P. Sambursky, Uma Mahesh Babu
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Patent number: 9121849Abstract: Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.Type: GrantFiled: March 8, 2013Date of Patent: September 1, 2015Assignee: Rapid Pathogen Screening, Inc.Inventors: Uma Mahesh Babu, Robert P. Sambursky, Robert W. VanDine
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Patent number: 9068981Abstract: A lateral flow device may include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone. In preferred embodiments, some or all of the enhancement elements are encapsulated.Type: GrantFiled: September 19, 2012Date of Patent: June 30, 2015Assignee: Rapid Pathogen Screening, Inc.Inventors: Uma Mahesh Babu, Robert W. VanDine, Robert P. Sambursky
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Publication number: 20150099666Abstract: A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device.Type: ApplicationFiled: December 19, 2014Publication date: April 9, 2015Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine
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Patent number: 8962260Abstract: A lateral flow assay is capable of detecting and differentiating viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In some preferred embodiments, bimodal methods and devices determine if an infection is bacterial and/or viral. A dual use two strip sample analysis device includes a first lateral flow chromatographic test strip to detect MxA and a low level of C-reactive protein and a second lateral flow chromatographic test strip to detect high levels of C-reactive protein. In some preferred embodiments, the sample is a fingerstick blood sample.Type: GrantFiled: March 8, 2013Date of Patent: February 24, 2015Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Robert W. VanDine, Uma Mahesh Babu, Peter Condon
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Patent number: 8822151Abstract: Point-of-care binding assays include at least one target nucleic acid binding in a multiplex structure with at least one sequence in a partner nucleic acid associated with a label, due to complementary base pairings between at least one sequence in the target nucleic acid and at least one sequence in the partner nucleic acid. The assays overcome the inherent deficiencies of antibody-protein antigen assays. In a preferred embodiment, color tagged nucleic acid sequences are used to bind a complementary target nucleic acid. The tagged nucleic acid sequences are preferably made from deoxyribonucleotides, ribonucleotides, or peptide nucleotides.Type: GrantFiled: June 25, 2012Date of Patent: September 2, 2014Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine
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Patent number: 8815609Abstract: A lateral flow device includes a sample compressor and a test strip comprising a diverting zone. The diverting zone, which may include a barrier and/or a gap or ditch, stops or impedes flow. Flow is reinitiated and diverted into an alternate plane by compression of a sample compressor. Flow returns to the original, lateral plane, at the end of the diverting zone.Type: GrantFiled: March 7, 2013Date of Patent: August 26, 2014Assignee: Rapid Pathogen Screening, Inc.Inventors: Uma Mahesh Babu, Robert P. Sambursky, Peter Condon, Robert W. VanDine
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Publication number: 20140227773Abstract: Methods and devices for detecting a target enzyme include an anchored or trapped peptide complex. The peptide complex includes an anchor particle immobilized on a sample analysis device or trapped in a reaction receptacle including a filter, a peptide, with at least one enzyme cleavage site for a target enzyme, bound to the anchor particle, at least one detectable label, and at least one first tag bound to the peptide on a side of the enzyme cleavage site opposite the anchor particle. When the target enzyme is present in the sample, the enzyme cleaves the peptide at the enzyme cleavage site, permitting the cleaved peptide to reach the test zone of a sample analysis device such that the first tag binds to the immobilized second tag and a signal is detected at the test zone.Type: ApplicationFiled: July 19, 2012Publication date: August 14, 2014Applicant: RAPID PATHOGEN SCREENING, INC.Inventors: Uma Mahesh Babu, Robert P. Sambursky, Robert W. VanDine
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Publication number: 20140199710Abstract: A sample compressor applies pressure to a sample collector and a sample application zone of a test strip to transfer a sample from the sample collector and a binding partner of an analyte to the sample application zone in a lateral flow device. At least one of the binding partners of the analyte is not located on the test strip prior to use of the lateral flow device. The test strip may be a universal test strip with no molecule that specifically binds the analyte is located on the test strip. The sample compressor may be a universal sample compressor also with no molecule that specifically binds the analyte on the sample compressor. The lateral flow device may also include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone.Type: ApplicationFiled: March 17, 2014Publication date: July 17, 2014Applicant: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine, Ganga V. Kanaujia, Thomas Orsini
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Patent number: 8669052Abstract: Point-of-care binding assays include at least one target nucleic acid binding in a multiplex structure with at least one sequence in a partner nucleic acid associated with a label, due to complementary base pairings between at least one sequence in the target nucleic acid and at least one sequence in the partner nucleic acid. The assays overcome the inherent deficiencies of antibody-protein antigen assays. In a preferred embodiment, color tagged nucleic acid sequences are used to bind a complementary target nucleic acid. The tagged nucleic acid sequences are preferably made from deoxyribonucleotides, ribonucleotides, or peptide nucleotides.Type: GrantFiled: July 14, 2009Date of Patent: March 11, 2014Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine
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Publication number: 20130344565Abstract: Full length MxA constructs and truncated MxA constructs produce human MxA protein in E. coli. The full length MxA and truncated MxA constructs are preferably E. coli codon-optimized to optimize the amount of protein made using the constructs. T5 or T7 promoters can each be used in combination with either the full length MxA or the truncated MxA constructs. In one preferred embodiment, the MxA protein produced by the full length MxA or truncated MxA constructs is used in a control prep or external control. In other preferred embodiments, the MxA protein is used as a therapeutic.Type: ApplicationFiled: March 7, 2013Publication date: December 26, 2013Applicant: RAPID PATHOGEN SCREENING, INC.Inventors: Robert W. VanDine, Robert P. Sambursky, Uma Mahesh Babu, Kyosuke Nagata
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Patent number: 8614101Abstract: Devices and methods incorporate lysis agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters a lysis agent. The lysis agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the initially lysis agent is localized between the sample application zone and the conjugate zone. The lysis agent is preferably soluble or miscible in the sample transport liquid, and the lysis agent is solubilized and activated upon contact with the sample transport liquid. The sample transport liquid then contains both lysis agent in solution or suspension and sample components in suspension. Any lysis-susceptible components in a sample, then being exposed in suspension to the lysis agent, are themselves lysed in situ. The running buffer then carries the analyte, including any lysis-freed components, to the detection zone.Type: GrantFiled: July 14, 2009Date of Patent: December 24, 2013Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert W. VanDine, Uma Mahesh Babu, Robert P. Sambursky
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Patent number: 8609433Abstract: A sample compressor applies pressure to a sample collector and a sample application zone of a test strip to transfer a sample from the sample collector and a binding partner of an analyte to the sample application zone in a lateral flow device. At least one of the binding partners of the analyte is not located on the test strip prior to use of the lateral flow device. The test strip may be a universal test strip with no molecule that specifically binds the analyte is located on the test strip. The sample compressor may be a universal sample compressor also with no molecule that specifically binds the analyte on the sample compressor. The lateral flow device may also include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone.Type: GrantFiled: December 2, 2010Date of Patent: December 17, 2013Assignee: Rapid Pathogen Screening, Inc.Inventors: Robert P. Sambursky, Uma Mahesh Babu, Robert W. VanDine, Ganga V. Kanaujia, Thomas Orsini
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Publication number: 20130295583Abstract: The present invention includes methods and devices for preventing interfering substances from affecting the accuracy of a lateral flow immunoassay. In preferred embodiments, a test strip includes a capturing zone that includes at least one mobile capturing reagent that separates at least one interfering substance from the analyte. The capturing zone is preferably located upstream of the sample application zone. In some embodiments, the reagent/conjugate zone is also located upstream of the sample application zone. The capturing zone may be located upstream, downstream, or overlapping with the reagent/conjugate zone in these embodiments. In other preferred embodiments, one or more mobile capturing reagents are included in the elution medium/running buffer. In yet other embodiments, the capturing reagent is incorporated into a sample collection device of a sample collection system, preferably separate from the chromatographic test strip. A lysis zone is also included in some preferred embodiments.Type: ApplicationFiled: March 13, 2013Publication date: November 7, 2013Inventors: Uma Mahesh Babu, Franz Aberl, Marcus Scheibenzuber, Robert P. Sambursky, Robert W. VanDine, Jose S. Sambursky
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Publication number: 20130230846Abstract: A lateral flow device includes a sample compressor and a test strip comprising a diverting zone. The diverting zone, which may include a barrier and/or a gap or ditch, stops or impedes flow. Flow is reinitiated and diverted into an alternate plane by compression of a sample compressor. Flow returns to the original, lateral plane, at the end of the diverting zone.Type: ApplicationFiled: March 7, 2013Publication date: September 5, 2013Applicant: RAPID PATHOGEN SCREENING, INC.Inventors: Uma Mahesh Babu, Robert P. Sambursky, Peter Condon, Robert W. VanDine