Abstract: A coupled nanomanipulation and nanospray mass spectrometry (NMS) system for single cell, single organelle, and ultra-trace molecular analysis is disclosed herein. The system primarily comprises a bio-workstation coupled to a NMS. The bio-workstation primarily comprises of a nanomanipulator stage with a plurality of nano-positioners attached to a cabinet with a piezo voltage source and a pressure injector. The present invention further describes a fingerprint lift method that when coupled with the system disclosed herein can be used for retrieval and analysis of trace amounts of drug and explosive residues. The system described herein has been used in the areas of trace and document analysis within the forensic field, trace fiber analysis, and electrostatic lifts for illicit drugs, as well as document and painting analysis.

Abstract: An apparatus and method for nonlinear conversion of laser light is described herein. The apparatus of the instant invention comprises a fiber coupled light source, a cavity in optical alignment with the first light beam from the light source, wherein the cavity comprises: two concave mirrors, one or more Brewster-cut periodically poled crystals, and a cavity servo to lock the length of cavity to laser frequency. In one aspect of the present invention the feedback to the lock is S-polarized component of first laser light reflected off the Brewster surface of nonlinear crystal.