Abstract: This invention relates to methods of expressing eukaryotic proteins in prokaryotic hosts, particularly eukaryotic proteins that require formation of disulfide bridges for biological activity. Various approaches are used including fusion to thioredoxin, cytoplasmic expression of disulfide isomerases, deficiencies in thioredoxin and/or glutathione reductases, deficiencies in proteases, and the like. The method is applicable to express monomeric and dimeric forms of the eukaryotic protein with biological activity such as monomeric and dimeric forms of a disintegrin or a disintegrin domain. Included are the vectors, host cells expressing the proteins, the expressed proteins and methods of using the proteins.

Abstract: A method for monitoring a control of a parameter of one or more devices or systems in an oil or gas production site includes receiving process data, the process data being a result of the control of the parameter of the one or more devices or systems in the production site; smoothing the process data using a polynomial filter while preserving features of the process data to obtain smoothed data; and applying a pattern recognition algorithm to the smoothed data to determine whether there is a malfunction condition in the one or more devices or systems.

Abstract: Various examples of feed-forward systems that reduce phase noise in a laser field generated by a laser. These include feed-forward systems that utilize phase and/or frequency discriminators, filters, integrators, voltage controlled oscillators (VCOs), current controlled oscillators (CCOs), phase modulators, and/or amplitude modulators. It also includes systems that use both feed-forward and feedback phase noise reduction systems, tunable semiconductor lasers, and multiple, sequential feed-forward systems.

Abstract: An organic light emitting device having an anode, a cathode and an organic layer disposed between the anode and the cathode is provided. In one aspect, the organic layer comprises a compound having at least one zwitterionic carbon donor ligand.

Abstract: Described microfluidic technology focused on: 1) direct integration of microfluidic devices with solidified liquid tissue samples, 2) subordination of architectural and operational principles of microfluidic devices specific tissue structure and needs and/or 3) on-chip sample acquisition integrated with the detection measurement within the same device. In contrast to conventional methods of off-chip sample prep and subsequent insertion into a detection device, new applications are possible on solidified liquid or solid tissue samples, such as in situ PCR.

Abstract: The present invention relates to drug derivatives and linkers. The invention specifically relates to compounds and methods of phosphonates and linkers, that are useful as carriers for imaging agents and useful in the treatment of various bone diseases.

Abstract: The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

Abstract: The invention provides methods for producing lipoxygenase inhibitors including the steps set forth in Schemes 2 and 3, and uses of the inhibitors produced by the methods set forth herein in to treat various disease states.

Abstract: Power consumed by a digital circuit that contains multiple sub-circuits may be computed. Each sub-circuit may include more than one transistor and have at least one input. A signal transition sensor may be connected to an input to each sub-circuit and configured to count the number of signal transitions at the input or to time the duration of a signal transition at the input. Weight information may be stored that is indicative of an amount of power that is being consumed by each sub-circuit based on a count that is counted by or a time duration that is timed by each signal transition sensor that is connected to an input of that sub-circuit. The amount of power being consumed by the digital circuit may be computed based on a count that is counted by or a time duration that is timed by each signal transition sensor and the weight information.

Abstract: A mobile software application may issue an alert when a user is not achieving a heath objective based on track movement of a mobile computer system that is running the application and being carried by the user and physical characteristics of the user. The software application may map information about the physical characteristics of the user to a user model and determine the energy being expended by the user based on the user model and movement information provided by the mobile computer system. The software application may determine the frequency of steps taken by the user by: bandpass filtering a time domain signal that is representative of the movement, transforming the filtered time domain signal into a frequency domain signal, and equating the step frequency with the frequency of the highest peak in the frequency domain signal.

Abstract: The present disclosure describes systems and techniques relating to generating three dimensional (3D) models from range sensor data. According to an aspect, 3D point clouds are captured using a 3D camera, where each of the 3D point clouds corresponds to a different relative position of the 3D camera with respect to a body. One of the 3D point clouds can be set as a reference point cloud, and transforms can be determined for coordinates of the other captured 3D point clouds to transform these to coordinates of the reference point cloud. The body represented in the reference point cloud can be segmented into body parts corresponding to elements of a 3D part-based volumetric model including cylindrical representations, and a segmented representation of the physical object of interest can be generated in accordance with the 3D part-based volumetric model, while taking localized articulated motion into account.

Abstract: Provided herein are compositions and methods for inhibiting viral infection of a host cell. The methods comprise contacting the the host cell with an effective amount of one or more polypeptides having a disintegrin domain. The polypeptide can be CN, VCN or modified ADAM-derived polypeptide (MAP), or a fusion protein comprising a CN, VCN or MAP.

Abstract: An autonomous pavement assessment system may receive depth data indicative of the depth of pixels that collectively comprise multiple defective areas of pavement. For each defective area, the system may fit a plane to it; generate a histogram that indicates the frequency of its pixels at different depths; dynamically determine a depth noise threshold for it; generate a binary image of it based on its noise threshold; and generate a depth map of it containing only the pixels that have a depth that meets or exceeds its depth noise threshold. The system may prioritize the multiple defective areas for repair and/or generate optimized rehabilitation routes. Crowd sourcing may be used to gather the depth data, as well as location information for each defective area.

Abstract: This disclosure relates to an in vivo treatment of a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 1 nanosecond at the full-width-half-maximum. This treatment may prevent at least growth of the lesion.

Abstract: The invention relates to a peptide of 8-50 amino acids comprising the sequence of KAHKKRAD or KARKKHAD, or a cyclic peptide of 8-50 amino acids comprising the sequence of HKKR or RKKH. Also disclosed are methods of using the peptide for detecting, monitoring, or treating cancer.

Abstract: Systems, devices, and methods are presented for a prosthetic injectable intraocular lens. The lenses can be made from silicone, fluorosilicone, and phenyl substituted silicone and be semipermeable to air. One or more silicone elastomeric patches located outside the optical path on the anterior side but away from the equator can be accessed by surgical needles in order to fill or adjust optically clear fluid within the lens. The fluid can be adjusted in order to set a base dioptric power of the lens and otherwise adjust a lens after its initial insertion. The elastomeric patches are sized so that they self-seal after a needle is withdrawn. A straight or stepped slit in the patch can allow a blunt needle to more easily access the interior of the lens.

Abstract: The present invention provides for scaffolds for growing RPE cells, comprising two or more biodegradable polymers. The present invention also provides for methods for creating a scaffold for growing RPE cells. Additionally, the present invention provides for RGD peptide linked polymer scaffolds for supporting the growth of RPE cells. The present invention provides methods of culturing RPE cells using the scaffolds produced herein. The present invention also provides methods of treating vision loss through the administration of RPE cell attached RGD peptide linked polymer scaffolds produced herein. The present invention further provides kits for treating vision loss.

Abstract: The present invention discloses methods and systems for analyzing microarray data. The method includes the general steps of providing microarray data, normalizing the data using a least trimmed squares regression, and then analyzing the normalized microarray data to obtain a desired result such as an expression profile. There is also disclosed a method of subdividing an array into subarrays before normalization. This approach provides a method for improving measurement accuracy and salvaging array data from arrays containing minor defects. Also disclosed is a Probe-Treatment-Reference (PTR) model for streamlining normalization and summarization of microarray data by allowing multiple references. Other aspects of the present invention include computer systems and computer readable media encoding methods of the present invention.

Abstract: Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 ?m to 5 ?m thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The application further provides an implantable cage-like apparatus for culturing cells comprising the parylene membrane.

Abstract: This application presents a bifurcated, optimally-angled surgical forceps. In one example, this surgical forceps may enable a more natural maneuver for initial clamping of the vas deferens through the scrotal skin. This may be more comfortable for users and easier to maintain, and may provide greater tactile surface contact between the thumb and vas deferens. This device also may provide the surgeon with an entire segment of vas deferens upon which the vasectomy may be performed, thus reducing the need for frequent repositioning of instruments. The device may also be applied to other surgical procedures that may benefit from the features of the device and where a section of a tubular anatomical structure may need clamping at two points along its length. Examples include blood and lymphatic vessels, ducts of the digestive system, and large nerves or nerve bundles.