Abstract: A reference device for evaluating the performance of a confocal laser scan microscope. The reference device comprises a substrate (53) and reference fluorescing matter distributed over a surface of the substrate (53). The reference fluorescing matter has a predetermined spatial distribution over the latter surface.

Abstract: A quality test of fluids of biological origin can be performed optically with applying a suitable evaluation method. In case of two components to be determined in a such fluid, an extinction spectrum is approximated in a first wavelength range by a combination of a merely theoretical curve and the spectrum of the pure first substance in a first wavelength range, and this evaluation is repeated in a second wavelength range this time by approximating the measured spectrum (62) by a combination of a hypothetical curve, the spectrum (64) of the first component with the already determined concentration, and the spectrum (65) of the pure second component. Furthermore, it is feasible to subtract the first and second spectrum and analyze the so obtained differential spectrum (66) in view of anomalies. The hypothetical curves are preferably straight lines which are defined by slope and ordinate section.

Abstract: A reference device for evaluating the performance of a confocal laser scan microscope. The reference device comprises a substrate (53) and reference fluorescing matter distributed over a surface of the substrate (53). The reference fluorescing matter has a predetermined spatial distribution over the latter surface.