Abstract: The present disclosure provides temperature sensitive essential nucleic acid molecules from a psychrophilic bacterium, proteins encoded by the nucleic acid molecules, as well as recombinant cells into which have been introduced such nucleic acid molecules. The disclosed recombinant cells containing one or more essential nucleic acid molecules from a psychrophilic bacterium are thereby made temperature sensitive, and can be administered to a mammal to induce an immune response in the mammal.

Abstract: An apparatus for measuring dimensions of a micro-scale object includes a movable stylus, a probe and at least one acoustic emissions sensor. The movable stylus is positionable to contact an object to be measured. The probe is rotatably coupled to the stylus and has a distal end that circumscribes a circle when rotated. The acoustic emissions sensor is operatively coupled to the probe. The sensor is operative to detect acoustic emissions generated from contact between the distal end of the probe and the object, thereby allowing dimensions of the object to be determined.