Abstract: The present invention relates to new compositions for isolating and/or stabilizing nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X? wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X? may represent an anion of an inorganic or organic, mono- or polybasic acid.

Abstract: New processes and equipment to isolate and purify nucleic acids on surfaces are provided. The invention focuses on processes which use surfaces, for example, porous membranes, on which the nucleic acids are immobilized in a simple manner from the sample containing the nucleic acids and can be released again by way of simple procedural steps, whereby the simple performance of the process according to the invention makes it possible to perform the processes specifically in a fully automatic manner. An additional aspect of the present invention focuses on binding the nucleic acids to an immobile phase, especially to a membrane, in such a way and manner, that they can be released without difficulty during an additional reaction stage from this phase and, if desired, can be used in other applications, such as restriction digestion, RT, PCR or RT-PCR, or in any of the suitable analyses or enzyme reactions mentioned in the disclosure.

Abstract: The present invention relates to new compositions for isolating and/or stabilising nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X? wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X? may represent an anion of an inorganic or organic, mono- or polybasic acid.

Abstract: The present invention provides RNA purification and/or analysis methods that use ammonium sulfate to mitigate or neutralize inhibitory effects of certain molecules. Exemplary inhibitory molecules that interfere with RNA function or analysis are those that bind to or cleave RNA, or stabilize RNA secondary structures.

Abstract: The present invention relates to the use of compositions for isolating and/or stabilizing nucleic acids in or from microorganisms—such as prokaryotes, fungi, protozoa or algae. The composition contains, as an essential ingredient, a cationic compound of general formula Y+R1R2R3R4X? wherein Y may denote nitrogen or phosphorus, R1, R2, R3 and R4 independently of one another may denote an unbranched or branched C1-C20-alkyl and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group, and X? may denote an anion of an inorganic or organic, mono- or polybasic acid.

Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.

Abstract: The present invention is directed to a method and device for collecting and stabilizing a biological sample, particularly a whole blood sample. More specifically, the present invention relates to the use of about 5.6 to about 37.5 mM, preferably about 5.6 to about 10.1 mM, EDTA during collection of the sample and to evacuated fluid sample containers having an amount of EDTA contained therein such that, when the sample is collected, the amount achieved is about 5.6 to about 37.5 mM, preferably about 5.6 to about 10.1 mM, EDTA to stabilize the sample.

Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.

Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes an effective amount of at least one stabilizing agent. The stabilizing agent is able to stabilize nucleic acids in the biological sample at the point of collection to prevent enzymatic degradation of the nucleic acids. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, and mixtures thereof.