Abstract: Disclosed are compositions comprising myokines and their methods of preparation and use. The disclosed myokine compositions and methods may comprise myokines having a molecular weight of greater than about 10 kDa such as myostatin and metrnl. The disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders. In particular, the disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders, such as cancer, and metabolic diseases and disorders, such as diabetes, non-alcoholic fatty liver disease, and heart disease.

Abstract: Disclosed are compositions comprising myokines and their methods of preparation and use. The disclosed myokine compositions and methods may comprise myokines having a molecular weight of greater than about 10 kDa such as myostatin and metrnl. The disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders. In particular, the disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders, such as cancer, and metabolic diseases and disorders, such as diabetes, non-alcoholic fatty liver disease, and heart disease.

Abstract: In an aspect, a method for imaging a target comprises steps of: performing optical coherence tomography (OCT) scanning on the target with one or more beams of source light, the one or more beams of source light comprising a plurality of wavelengths; wherein performing OCT scanning comprises: providing the source light to a reference optical path and to a sample optical path, wherein providing the source light to a sample optical path comprises illuminating the target with the source light; and recording interference data corresponding to an interaction of a light from the reference optical path and a light from the sample optical path; processing the interference data; and identifying blood or one or more blood-features in the target based on an optical attenuation of light in or associated with the sample optical path by the blood or the one or more blood-features.

Abstract: In an aspect, a method for imaging a target comprises steps of: performing optical coherence tomography (OCT) scanning on the target with one or more beams of source light, the one or more beams of source light comprising a plurality of wavelengths; wherein performing OCT scanning comprises: providing the source light to a reference optical path and to a sample optical path, wherein providing the source light to a sample optical path comprises illuminating the target with the source light; and recording interference data corresponding to an interaction of a light from the reference optical path and a light from the sample optical path; processing the interference data; and identifying blood or one or more blood-features in the target based on an optical attenuation of light in or associated with the sample optical path by the blood or the one or more blood-features.

Abstract: Low-coherence enhanced backscattering (LEBS) spectroscopy is an angular resolved backscattering technique that is sensitive to sub-diffusion light transport length scales in which information about the scattering phase function is preserved. Lens-based and lens-free fiber optic LEBS probes are described that are capable of measuring optical properties of a target tissue through depth-limited measurements of backscattering angles within the enhanced backscattered cone.

Abstract: An automated calibration system that includes a probe guide and a target assembly. The probe guide receives an optical probe, and the target assembly includes one or more calibration targets. The target assembly is slideable relative to the probe guide so that a first calibration target is aligned under the optical probe in a first position of the target assembly and a second calibration target is aligned under the optical probe in a second position of the target assembly.

Abstract: Methods and apparatus initiate a procedure performed by a probe on tissue. A signal is obtained from the probe, the signal varying with changes in a proximity of the probe to the tissue. The obtained signal is analyzed by a controller to determine whether the probe is within a predetermined distance of the tissue. The controller initiates the procedure when it is determined that the probe is within the predetermined distance of the tissue. The obtaining and analyzing may be repeated until the probe is determined to be within the predetermined distance of the tissue. The analyzing may include comparing the obtained signal to a predetermined threshold.

Abstract: A pre-fabricated, on-demand interface provides optical coupling between disposable and reusable portions of a fiber-optic probe. The interface uses a pre-cured, compressible optically-transmissive gel in a cavity of the disposable portion, which can be a disposable insertion tip of the fiber optic probe. The disposable portion includes an elongated housing, or sheath, and the cavity is located within and proximal of a distal end of the elongated housing. A proximal end of an optical assembly of the disposable portion is located within the cavity and contacts the gel. A first coupling, provided at a proximal end of the elongated housing, detachably couples to a mating, second coupling of the probe reusable portion. When the disposable and reusable portions are coupled together, the gel is compressed between the proximal end of the optical assembly of the disposable portion and a distal optical member of an optical assembly of the reusable portion.

Abstract: Disclosed are compositions comprising myokines and their methods of preparation and use. The disclosed myokine compositions and methods may comprise myokines having a molecular weight of greater than about 10 kDa such as myostatin and metrnl. The disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders. In particular, the disclosed myokine compositions and methods may be utilized for treating and/or preventing cell proliferative and metabolic diseases and disorders, such as cancer, and metabolic diseases and disorders, such as diabetes, non-alcoholic fatty liver disease, and heart disease.

Abstract: Provided herein are chromatin protection therapeutics (CPTs) and methods of targeting chromatin heterogeneity for the treatment of cancer therewith. In particular compositions and methods are provided that target physical variations in chromatin topology, reduce chromatic heterogeneity, and treat cancer or inhibit the development of resistance to other cancer therapeutics.

Abstract: Low-coherence enhanced backscattering (LEBS) spectroscopy is an angular resolved backscattering technique that is sensitive to sub-diffusion light transport length scales in which information about the scattering phase function is preserved. Lens-based and lens-free fiber optic LEBS probes are described that are capable of measuring optical properties of a target tissue through depth-limited measurements of backscattering angles within the enhanced backscattered cone.

Abstract: The present invention relates to detection of cancer, or assessment of risk of development thereof. In particular, the present invention provides compositions and methods detection of field carcinogenesis by identification of ultrastructural and molecular markers in a subject.

Abstract: A sample collection apparatus or device, various abrasive devices or components, and methods for their use, are provided for collecting samples from within a mammalian body. Tissue collection and sample preparation is the gold standard in cytology. Many cell scrapers and abrasives and their respective methods of collection destroy cellular tissue, decreasing the quality of the sample. Devices for obtaining cell and tissue samples, can comprise a collection device which is inserted into a cavity, a finger worn collection device, a glove based collection device and respective abrasive instruments or components.

Abstract: The present invention provides systems, devices, and methods for cell collection and/or cell smearing (e.g., on a slide). In particular, provided herein is an cell collection device, a translating slide holder, systems comprising such devices, and methods of use thereof.

Abstract: A method and system to measure and image the full optical scattering properties by inverse spectroscopic optical coherence tomography (ISOCT) is disclosed. Tissue is modeled as a medium with continuous refractive index (RI) fluctuation and such a fluctuation is described by the RI correlation functions. By measuring optical quantities of tissue (including the scattering power of the OCT spectrum, the reflection albedo ? defined as the ratio or scattering coefficient ?s, and the back-scattering coefficient ?b), the RI correlation function can be inversely deduced and the full set of optical scattering properties can be obtained.

Abstract: The present technology provides methods, systems, and apparatuses to achieve high throughput and high speed acquisition of partial wave spectroscopic (PWS) microscopic images. In particular, provided herein are high-throughput, automated partial wave spectroscopy (HT/A-PWS) instruments and systems capable of rapid acquisition of PWS Microscopic images and clinical, diagnostic, and research applications thereof.

Abstract: An automated calibration system that includes a probe guide and a target assembly. The probe guide receives an optical probe, and the target assembly includes one or more calibration targets. The target assembly is slideable relative to the probe guide so that a first calibration target is aligned under the optical probe in a first position of the target assembly and a second calibration target is aligned under the optical probe in a second position of the target assembly.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Low-coherence enhanced backscattering (LEBS) spectroscopy is an angular resolved backscattering technique that is sensitive to sub-diffusion light transport length scales in which information about the scattering phase function is preserved. Lens-based and lens-free fiber optic LEBS probes are described that are capable of measuring optical properties of a target tissue through depth-limited measurements of backscattering angles within the enhanced backscattered cone.

Abstract: The present invention relates to detection of cancer, or assessment of risk of development thereof. In particular, the present invention provides compositions and methods detection of field carcinogenesis by identification of ultrastructural and molecular markers in a subject.