Abstract: Disclosed herein is a method for producing an exosome containing therapeutic agent, including: separating mononuclear cells from a bone marrow sample of a healthy donor; treating the separated mononuclear cells in a culture media at 37° C. and at 5% CO2 for 48-72 h; separating an exosome composition from the culture media; and dissolving the separated exosome composition in a phosphate buffer to obtain the exosome containing therapeutic agent. The culture media includes a 1:1 mixture of DMEM/F12 and HEPES, 2 mmol/mL or 3.65 mg/10 mL of L-glutamine, 100 units/mL of penicillin, 100 ?g/mL of streptomycin, and 10 wt % of fetal bovine serum.

Abstract: Provided herein is a substance for use in a therapeutic method for stimulating cells in the human immune system, a method for producing this substance, and a method of use of the substance for stimulating cells of the human immune system in treating various diseases involving immune system disorders.

Abstract: Provided herein is a substance for use in a therapeutic method for stimulating cells in the human immune system, a method for producing this substance, and a method of use of the substance for stimulating cells of the human immune system in treating various diseases involving immune system disorders.

Abstract: Disclosed herein is a method for producing an exosome containing therapeutic agent, including: separating tumor cells from a biomaterial sample of a subject; treating the separated tumor cells in a culture media at 37° C. and at 5% CO2 for 24-72 h; separating an exosome composition from the culture media; and dissolving the separated exosome composition in a phosphate buffer to obtain the exosome containing therapeutic agent; characterized in that the culture media comprises either (i) a 1:1 mixture of DMEM/F12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 2 mmol/mL or 3.65 mg/10 mL of L-glutamine, 100 units/mL of penicillin, 100 ?g/mL of streptomycin, and 10 wt % of fetal bovine serum; or (ii) 79 wt % of RPMI, 20 wt % of fetal bovine serum and 1 wt % of streptomycin.

Abstract: Provided herein is a substance for use in a therapeutic method for stimulating cells in the human immune system, a method for producing this substance, and a method of use of the substance for stimulating cells of the human immune system in treating various diseases involving immune system disorders.

Abstract: Disclosed herein is a method for producing an exosome containing therapeutic agent, including: separating tumor cells from a biomaterial sample of a subject; treating the separated tumor cells in a culture media at 37° C. and at 5% CO2 for 24-72 h; separating an exosome composition from the culture media; and dissolving the separated exosome composition in a phosphate buffer to obtain the exosome containing therapeutic agent; characterized in that the culture media comprises either (i) a 1:1 mixture of DMEM/F12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 2 mmol/mL or 3.65 mg/10 mL of L-glutamine, 100 units/mL of penicillin, 100 ?g/mL of streptomycin, and 10 wt % of fetal bovine serum; or (ii) 79 wt % of RPMI, 20 wt % of fetal bovine serum and 1 wt % of streptomycin.

Abstract: Disclosed herein is a method for producing an exosome containing therapeutic agent, including: separating mononuclear cells from a bone marrow sample of a healthy donor; treating the separated mononuclear cells in a culture media at 37° C. and at 5% CO2 for 48-72 h; separating an exosome composition from the culture media; and dissolving the separated exosome composition in a phosphate buffer to obtain the exosome containing therapeutic agent. The culture media includes a 1:1 mixture of DMEM/F12 and HEPES, 2 mmol/mL or 3.65 mg/10 mL of L-glutamine, 100 units/mL of penicillin, 100 ?g/mL of streptomycin, and 10 wt % of fetal bovine serum.