Abstract: Systems and methods for compressing images that include a memory storing an executable code and a processor executing the code to receive a whole slide image, the whole slide image containing a plurality of image layers and metadata associated with each image layer, extract a high-resolution image layer and the corresponding metadata, wherein the high-resolution image layer includes a plurality of image tiles including informative tiles and noninformative tiles, where the informative tiles depict a region of interest of the specimen, analyze the image tiles of the extracted high-resolution image layer, determine a first tile is a noninformative tile, create an informative image layer by removing the first tile from the extracted high-resolution image layer, the informative image layer containing a plurality of informative tiles, compress the informative image layer into a single-layer whole slide image, and save the single-layer whole slide image in the memory.