Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.

Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.

Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.

Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.

Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.

Abstract: A multiple promoter platform for expression of a protein or multiple and different proteins in a microorganism comprising two or more expression vectors having a nucleic acid molecule encoding a protein to be expressed, or having different nucleic acid molecules encoding different proteins to be expressed, wherein each vector has a different promoter operably linked to the nucleic acid molecule, and uses of the multiple promoter platform to produce recombinant proteins.

Abstract: The invention is directed to a method for obtaining a promoter from an organism using a proteomics approach, isolated promoters from fungal species and uses of the isolated promoters.