Patents by Inventor Valerio Pini
Valerio Pini has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240133881Abstract: Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.Type: ApplicationFiled: October 19, 2023Publication date: April 25, 2024Inventors: Valerio Pini, Adonis Stassinopoulos, Matthias Mösl, Jesús Óscar Ahumada Heredero, Chloé Alexia Rodriguez, Andreas Thon, Houman Yaghoubi, Scott Rongey
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Patent number: 11519856Abstract: A method for optically detecting biomarkers in a biosensor is disclosed, wherein the optical detection obtains spatially and spectrally resolved optical signals from a sample on a biosensor, and one or more of these spatially and spectrally resolved optical signals can be analyzed in parallel with image acquisition. The image analysis comprises reading data of the acquired images, correcting them to reduce inhomogeneities and noise, localizing particles in the images, characterizing each particle individually to obtain its position and characterization parameters, and classifying the particles based on their characterization parameters. Using the number of particles per class for all the acquired images of the sample, a statistical value is calculated per sample and each statistical value is correlated with an indication of the presence of a biomarker in the sample.Type: GrantFiled: April 3, 2020Date of Patent: December 6, 2022Assignee: Mecwins, S.A.Inventors: Andreas Thon, Valerio Pini, Antonio Salvador-Matar Renteria, Virginia Cebrián Hernando, Carlos García Aguado, Jesús Oscar Ahumada Heredero
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Patent number: 11519843Abstract: Biosensing platform for simultaneous, multiplexed, high throughput and ultra-sensitive optical detection of biomarkers labelled with plasmonic nanoparticles, the platform being provided with a biosensor, a broadband and continuous spectrum illumination source, an optical detector for simultaneously capturing spatially resolved and spectrally resolved the scattering signal of each individual nanoparticle, an autofocus system and an optical system adapted to collect the scattered signal of the biosensor's surface onto the optical detector, the platform being provided with translation means for the optical system and/or the biosensor, such that the optical system and the biosensor can be displaced relative to each other in the three dimensions, and wherein the processing means are adapted to: i) simultaneously capture spatially and spectrally resolved scattering signals from each nanoparticle individually, and ii) to analyze these signals simultaneously with the capture process.Type: GrantFiled: April 3, 2020Date of Patent: December 6, 2022Assignee: MECWINS, S.A.Inventors: Valerio Pini, Andreas Thon, Antonio Salvador-Matar Renteria, Virginia Cebrián Hernando, Carlos García Aguado, Jesús Oscar Ahumada Heredero
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Publication number: 20200319085Abstract: Biosensing platform for simultaneous, multiplexed, high throughput and ultra-sensitive optical detection of biomarkers labelled with plasmonic nanoparticles, the platform being provided with a biosensor, a broadband and continuous spectrum illumination source, an optical detector for simultaneously capturing spatially resolved and spectrally resolved the scattering signal of each individual nanoparticle, an autofocus system and an optical system adapted to collect the scattered signal of the biosensor's surface onto the optical detector, the platform being provided with translation means for the optical system and/or the biosensor, such that the optical system and the biosensor can be displaced relative to each other in the three dimensions, and wherein the processing means are adapted to: i) simultaneously capture spatially and spectrally resolved scattering signals from each nanoparticle individually, and ii) to analyze these signals simultaneously with the capture process.Type: ApplicationFiled: April 3, 2020Publication date: October 8, 2020Inventors: Valerio PINI, Andreas THON, Antonio SALVADOR-MATAR RENTERIA, Virginia CEBRIÁN HERNANDO, Carlos GARCÍA AGUADO, Jesús Oscar AHUMADA HEREDERO
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Publication number: 20200319102Abstract: A method for optically detecting biomarkers in a biosensor, comprising: simultaneously acquiring (1100) spatially and spectrally resolved images from at least one sample of the biosensor and performing an image analysis (1000) in parallel to the image acquisition (1100); wherein the image analysis (1000) comprises: reading (2100) data of the acquired images; correcting (2200) the data to reduce inhomogeneities and noise of the images; localizing (2300) particles in the images using the corrected data; characterizing (2400) each particle individually to obtain at least its position and characterization parameters; classifying (2500) the particles based on their characterization parameters to obtain particle classes; counting (2600) the particles for each class and acquired image; for each biomarker in each sample, calculating an overall analysis result (2800) comprising calculating at least one statistical value by using the number of particles per class for all the images acquired from the same sample, anType: ApplicationFiled: April 3, 2020Publication date: October 8, 2020Inventors: Andreas THON, Valerio PINI, Antonio SALVADOR-MATAR RENTERIA, Virginia CEBRIÁN HERNANDO, Carlos GARCÍA AGUADO, Jesús Oscar AHUMADA HEREDERO
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Publication number: 20200072829Abstract: The present invention relates to a system for biodetection applications comprising two basic elements, a substrate with a functionalized surface and a nanoparticle, the system being capable of enhancing the plasmonic effect of the nanoparticle. The invention also relates to a biosensor incorporating such system, in addition to the method for detecting and quantifying a target analyte selected in a sample using such system. Finally, the invention relates to a device which can detect the enhanced optoplasmonic effect of the nanoparticles by means of the system of the invention or by combining the detection of such optoplasmonic effect with the analysis of the changes in the mechanical characteristics in the substrate.Type: ApplicationFiled: October 30, 2019Publication date: March 5, 2020Inventors: Francisco Javier TAMAYO DE MIGUEL, Priscila MONTEIRO KOSAKA, Valerio PINI, Montserrat CALLEJA GOMÉZ, Jose Jaime RUZ MARTINEZ, Daniel RAMOS VEGA, Maria Ujue GONZALEZ SAGARDOY
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Patent number: 10502734Abstract: The present invention relates to a system for biodetection applications comprising two basic elements, a substrate with a functionalized surface and a nanoparticle, the system being capable of enhancing the plasmonic effect of the nanoparticle. The invention also relates to a biosensor incorporating such system, in addition to the method for detecting and quantifying a target analyte selected in a sample using such system. Finally, the invention relates to a device which can detect the enhanced optoplasmonic effect of the nanoparticles by means of the system of the invention or by combining the detection of such optoplasmonic effect with the analysis of the changes in the mechanical characteristics in the substrate.Type: GrantFiled: June 2, 2015Date of Patent: December 10, 2019Assignee: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASInventors: Francisco Javier Tamayo de Miguel, Priscila Monteiro Kosaka, Valerio Pini, Montserrat Calleja Goméz, Jose Jaime Ruz Martinez, Daniel Ramos Vega, Maria Ujue Gonzalez Sagardoy
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Patent number: 10281330Abstract: The invention relates to a spectrophotometer, especially a spectrophotometer that can carry out simultaneous analysis at different points on the same sample (4), with a high spatial resolution and without requiring a mechanical system for physical scanning along the sample. This is obtained by the provision of means for processing the light received by the photodetectors (5), said processing means having a correlation wherein each of the photodetectors (5) corresponds to a spatial point on the sample (4). In the case of dark field applications, the present invention ensures the standardization of the data using the same measure.Type: GrantFiled: October 8, 2015Date of Patent: May 7, 2019Assignee: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASInventors: Valerio Pini, Priscila Monteiro Kosaka, Francisco Javier Tamayo De Miguel, Montserrat Calleja Gomez, Daniel Ramos Vega, Oscar Malvar Vidal, Jose Jaime Ruz Martinez, Mario Encinar Del Pozo
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Publication number: 20180017444Abstract: The invention relates to a spectrophotometer, especially a spectrophotometer that can carry out simultaneous analysis at different points on the same sample (4), with a high spatial resolution and without requiring a mechanical system for physical scanning along the sample. This is obtained by the provision of means for processing the light received by the photodetectors (5), said processing means having a correlation wherein each of the photodetectors (5) corresponds to a spatial point on the sample (4). In the case of dark field applications, the present invention ensures the standardization of the data using the same measure.Type: ApplicationFiled: October 8, 2015Publication date: January 18, 2018Inventors: Valerio PINI, Priscila MONTEIRO KOSAKA, Francisco Javier TAMAYO DE MIGUEL, Montserrat CALLEJA GOMEZ, Daniel RAMOS VEGA, Oscar MALVAR VIDAL, Jose Jaime RUZ MARTINEZ, Mario ENCINAR DEL POZO
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Patent number: 9829427Abstract: Method and system in optical microscopy based on the deflection of micro- and nanomechanical structures, upon impact of a laser beam thereon, which simultaneously and automatically provides a spatial map of the static deflection and of the form of various vibration modes, with vertical resolution in the subangstrom range. The invention comprises at least one mechanical structure, an incident laser beam sweeping the surface of the structure, an optometric detector for capturing the laser beam, and frequency excitation means that generate at least two sinusoidal signals at different frequencies in the mechanical structure.Type: GrantFiled: May 23, 2013Date of Patent: November 28, 2017Assignee: Consejo Superior De Investigaciones Cientificas (CSIC)Inventors: Francisco Javier Tamayo De Miguel, Valerio Pini, Priscila Monteiro Kosaka, Montserrat Calleja Gomez, Sheila Gonzalez Castilla
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Publication number: 20170205405Abstract: The present invention relates to a system for biodetection applications comprising two basic elements, a substrate with a functionalized surface and a nanoparticle, the system being capable of enhancing the plasmonic effect of the nanoparticle. The invention also relates to a biosensor incorporating such system, in addition to the method for detecting and quantifying a target analyte selected in a sample using such system. Finally, the invention relates to a device which can detect the enhanced optoplasmonic effect of the nanoparticles by means of the system of the invention or by combining the detection of such optoplasmonic effect with the analysis of the changes in the mechanical characteristics in the substrate.Type: ApplicationFiled: June 2, 2015Publication date: July 20, 2017Inventors: Francisco Javier TAMAYO DE MIGUEL, Priscila MONTEIRO KOSAKA, Valerio PINI, Montserrat CALLEJA GOMÉZ, Jose Jaime RUZ MARTINEZ, Daniel RAMOS VEGA, Maria Ujue GONZALEZ SAGARDOY
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Publication number: 20150177126Abstract: Method and system in optical microscopy based on the deflection of micro- and nanomechanical structures, upon impact of a laser beam theron, which simultaneously and automatically provides a spatial map of the static deflection and of the form of various vibration modes, with vertical resolution in the subangstrom range. The invention comprises at least one mechanical structure, an incident laser beam sweeping the surface of the structure, an optometric detector for capturing the laser beam, and frequency excitation means that generate at least two sinusoidal signals at different frequencies in the mechanical structure.Type: ApplicationFiled: May 23, 2013Publication date: June 25, 2015Inventors: Francisco Javier Tamayo De Miguel, Valerio Pini, Priscila Monteiro Kosaka, Montserrat Calleja Gomez, Sheila Gonzalez Castilla