Abstract: A method of manufacturing a stem cell sheets is provided which includes: (a) obtaining mesenchymal stem cells; (b) extracting programmed death ligands one (PD-L1) from the mesenchymal stem cells; (c) selecting only PD-L1 positive (PD-L1+ MSCs) from the PD-L1; (d) differentiating the PD-L1+ cells into osteoblasts and chondroblasts in a predetermined activation condition; and (e) forming the stem cell sheets by mixing the PD-L1+ MSCs with platelet rich plasma solution and CaCl2).

Abstract: A recombinant vector according to an embodiment is for genome editing without inserting a replicon into the plant genome in a T0 generation plant. The recombinant vector includes a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid. A method of genome editing without inserting a replicon into the plant genome in a T0 generation plant according to an embodiment includes transforming a plant cell by inserting a foreign gene to the aforementioned recombinant vector.

Abstract: A system includes a first structure configured to be coupled to a tubular string extending to a seafloor, whereby the first structure comprises a drill floor. The system further includes a second structure configured to provide a lateral force to the first structure while allowing for vertical movement between the first structure and the second structure relative to the seafloor.

Abstract: A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.

Abstract: Methods and systems for preparing acetone from cumene hydroperoxide (CHP) are disclosed. The disclosed methods involve cleaving CHP to form a cleavage product stream. In some embodiments, the cleavage product stream is separated into an overhead stream and a bottoms stream. The bottoms stream is neutralized, washed and then treated in a crude acetone column to provide a crude acetone stream. The overhead stream of the cleavage product is flashed forward in the process, bypassing the neutralization, washing, and crude acetone column and is then combined with the crude acetone stream. The combined acetone streams are provided to an acetone product column. According to some embodiments, the acetone product column comprises a side draw for obtaining a recycle acetone stream, which is recycled to the cleavage reactor(s). The recycle acetone side draw may be located lower on the acetone product column than the point from which product acetone is obtained.

Abstract: A recombinant vector according to an embodiment is for genome editing without inserting a replicon into the plant genome in a T0 generation plant. The recombinant vector includes a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid. A method of genome editing without inserting a replicon into the plant genome in a T0 generation plant according to an embodiment includes transforming a plant cell by inserting a foreign gene to the aforementioned recombinant vector.

Abstract: Methods and systems for preparing acetone from cumene hydroperoxide (CHP) are disclosed. The disclosed methods involve cleaving CHP to form a cleavage product stream. In some embodiments, the cleavage product stream is separated into an overhead stream and a bottoms stream. The bottoms stream is neutralized, washed and then treated in a crude acetone column to provide a crude acetone stream. The overhead stream of the cleavage product is flashed forward in the process, bypassing the neutralization, washing, and crude acetone column and is then combined with the crude acetone stream. The combined acetone streams are provided to an acetone product column. According to some embodiments, the acetone product column comprises a side draw for obtaining a recycle acetone stream, which is recycled to the cleavage reactor(s). The recycle acetone side draw may be located lower on the acetone product column than the point from which product acetone is obtained.

Abstract: A device includes a first cavity sized to hold a first cassette of tubulars, a second cavity sized to hold a second cassette of tubulars, and a first lifting mechanism disposed in the first cavity. The first lifting mechanism when in operation lifts the first cassette of tubulars from a horizontal orientation to a vertical orientation with respect to a drill floor. The device also includes a second lifting mechanism disposed in the second cavity, wherein the second lifting mechanism when in operation lifts the second cassette of tubulars from the horizontal orientation to the vertical orientation and a drive mechanism that when in operation rotates the device by a predetermined amount to dispose the first cavity in a region directly proximate to the drill floor.

Abstract: Methods and systems for preparing acetone from cumene hydroperoxide (CHP) are disclosed. The methods and systems involve an acetone purification column configured to separate acetone from other components of the product/reactant stream. The acetone purification column is equipped with a side draw configured to route a portion of the column contents to a caustic treating vessel where the contents are reacted with a dilute aqueous alkaline solution to remove aldehydes from the acetone product. The contents of the caustic treating vessel are then routed back to the acetone purification column. Using a separate caustic treating vessel for the aldehydes removal provides increased contact time between the acetone product and the alkaline solution, thereby affording greater aldehydes removal.

Abstract: A system includes a first structure configured to be coupled to a tubular string extending to a seafloor, whereby the first structure comprises a drill floor. The system further includes a second structure configured to provide a lateral force to the first structure while allowing for vertical movement between the first structure and the second structure relative to the seafloor.

Abstract: A device includes a first cavity sized to hold a first cassette of tubulars, a second cavity sized to hold a second cassette of tubulars, and a first lifting mechanism disposed in the first cavity. The first lifting mechanism when in operation lifts the first cassette of tubulars from a horizontal orientation to a vertical orientation with respect to a drill floor. The device also includes a second lifting mechanism disposed in the second cavity, wherein the second lifting mechanism when in operation lifts the second cassette of tubulars from the horizontal orientation to the vertical orientation and a drive mechanism that when in operation rotates the device by a predetermined amount to dispose the first cavity in a region directly proximate to the drill floor.

Abstract: A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.

Abstract: Techniques and systems to remove a portion of a drill floor from an offshore vessel. The drill floor may include an immovable section comprising a first portion of a surface area of the drill floor. The drill floor may also include a moveable section comprising a second portion of the surface area of the drill floor. Additionally, the drill floor may include an actuator configured to retract the moveable section from a first position to a second position to remove the second portion of the surface area of the drill floor.

Abstract: Techniques and systems to reduce movement of at least one portion of an offshore platform. One portion of the offshore platform can provide a connection to a seafloor. A second portion of the offshore platform provides a lateral force to the first portion of the offshore platform while allowing for vertical movement between the first portion and the second portion of the offshore platform.

Abstract: Techniques and systems to store and deploy a lift frame of an offshore vessel. A device may include a member arm comprising a locking feature configured to couple the member arm to a lift frame of the offshore vessel. The device may also include a base configured to be coupled to a drill floor of the offshore vessel, wherein the base comprises a joint configured to allow for rotation of the member arm and the lift frame from a storage position having a first angle between the member arm and the drill floor and a deployment position having a second angle between the member arm and the drill floor.

Abstract: Techniques and systems to remove a portion of a drill floor from an offshore vessel. The drill floor may include an immovable section comprising a first portion of a surface area of the drill floor. The drill floor may also include a moveable section comprising a second portion of the surface area of the drill floor. Additionally, the drill floor may include an actuator configured to retract the moveable section from a first position to a second position to remove the second portion of the surface area of the drill floor.

Abstract: Techniques and systems to store and deploy a lift frame of an offshore vessel. A device may include a member arm comprising a locking feature configured to couple the member arm to a lift frame of the offshore vessel. The device may also include a base configured to be coupled to a drill floor of the offshore vessel, wherein the base comprises a joint configured to allow for rotation of the member arm and the lift frame from a storage position having a first angle between the member arm and the drill floor and a deployment position having a second angle between the member arm and the drill floor.

Abstract: Techniques and systems to reduce movement of at least one portion of an offshore platform. One portion of the offshore platform can provide a connection to a seafloor. A second portion of the offshore platform provides a lateral force to the first portion of the offshore platform while allowing for vertical movement between the first portion and the second portion of the offshore platform.

Abstract: An apparatus for molding articles includes a first mold carrier configured to angularly displace relative to a fixed structure from a first molding position to first accessing position and a second mold carrier traveling with the first mold carrier and configured to angularly displace relative to the first mold carrier from a second molding position to a second accessing position. The first mold carrier may be pivotably coupled to the fixed structure; the second mold carrier may be slidably and rotatably coupled to the first mold carrier. A third mold carrier may be provided, and the second mold carrier may be located between the first and third mold carriers. An articulation mechanism may be provided to control relative motion of the second mold carrier relative to the first mold carrier as the first mold carrier pivots relative to the fixed structure. A method for molding articles is also provided.

Abstract: An apparatus for molding articles includes a first mold carrier configured to angularly displace relative to a fixed structure from a first molding position to first accessing position and a second mold carrier traveling with the first mold carrier and configured to angularly displace relative to the first mold carrier from a second molding position to a second accessing position. The first mold carrier may be pivotably coupled to the fixed structure; the second mold carrier may be slidably and rotatably coupled to the first mold carrier. A third mold carrier may be provided, and the second mold carrier may be located between the first and third mold carriers. An articulation mechanism may be provided to control relative motion of the second mold carrier relative to the first mold carrier as the first mold carrier pivots relative to the fixed structure. A method for molding articles is also provided.