Patents by Inventor Vanda White
Vanda White has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220170065Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: ApplicationFiled: November 8, 2021Publication date: June 2, 2022Applicant: BECTON DICKINSON AND COMPANYInventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
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Patent number: 11225681Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.Type: GrantFiled: November 7, 2019Date of Patent: January 18, 2022Assignee: BECTON, DICKINSON AND COMPANYInventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
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Patent number: 11193158Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: GrantFiled: January 6, 2020Date of Patent: December 7, 2021Assignee: BECTON, DICKINSON AND COMPANYInventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
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Publication number: 20200149085Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: ApplicationFiled: January 6, 2020Publication date: May 14, 2020Inventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
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Publication number: 20200087702Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.Type: ApplicationFiled: November 7, 2019Publication date: March 19, 2020Applicant: BECTON DICKINSON AND COMPANYInventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
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Patent number: 10557162Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: GrantFiled: March 29, 2017Date of Patent: February 11, 2020Assignee: BECTON, DICKINSON AND COMPANYInventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo
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Patent number: 10519482Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.Type: GrantFiled: February 28, 2013Date of Patent: December 31, 2019Assignee: Becton, Dickinson And CompanyInventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
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Publication number: 20170204448Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: ApplicationFiled: March 29, 2017Publication date: July 20, 2017Inventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo, Mary R. Votta, Ben Turng, Donald R. Callihan, Wendy Louise Williams
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Patent number: 9631221Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.Type: GrantFiled: August 31, 2012Date of Patent: April 25, 2017Assignee: Becton, Dickinson and CompanyInventors: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien Malick, Jon E. Salomon, John D. Mantlo
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Publication number: 20150125895Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.Type: ApplicationFiled: February 28, 2013Publication date: May 7, 2015Applicant: Becton, Dickinson and CompanyInventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
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Patent number: 8603769Abstract: Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of S. pneumoniae.Type: GrantFiled: October 8, 2012Date of Patent: December 10, 2013Assignee: Becton, Dickinson and CompanyInventors: Liping Feng, William B. Brasso, Song Shi, Ben Turng, Susan M. Kircher, Vanda White, Dyan Luper, Julie Rosales, Gretta Campbell, Adrien Malick