Patents by Inventor Vasiliy Nikolaevich Goral

Vasiliy Nikolaevich Goral has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200255783
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 13, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E. Romeo
  • Publication number: 20200248121
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 6, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E Romeo
  • Publication number: 20200248120
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 6, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E Romeo
  • Publication number: 20200248122
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 6, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E. Romeo
  • Publication number: 20200248123
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 6, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E. Romeo
  • Publication number: 20200248124
    Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
    Type: Application
    Filed: February 4, 2020
    Publication date: August 6, 2020
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori E. Romeo
  • Publication number: 20200181553
    Abstract: A cell culture vessel (400) includes a base defining a base plane extending in a first direction and a second direction perpendicular to the first direction, a plurality of cell culture chambers (200) stacked one atop another, each cell culture chamber having a top (202), a bottom (201) and sidewalls (203), each of the top, bottom and sidewalls having an interior surface, wherein at least the bottom surface has an array of microcavities (420) supporting the culture of cells as spheroids and each bottom surface is at an angle with respect to the plane of a table or surface upon which the vessel sits. Further, liquid can flow into each cell culture chamber via an inlet (210) and out of each cell culture chamber via an outlet (215). The angled cell culture surface allows the cell culture chambers to be perfused or allows media changes without dislodging spheroids from microcavities.
    Type: Application
    Filed: July 13, 2018
    Publication date: June 11, 2020
    Inventor: Vasiliy Nikolaevich Goral
  • Publication number: 20200148989
    Abstract: A cell culture vessel (100) has walls and a substrate having a plurality of microcavities (120), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange (170) surrounds the substrate having an array of microcavities. A channel (175, 176) surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.
    Type: Application
    Filed: July 13, 2018
    Publication date: May 14, 2020
    Applicant: CORNING INCORPORATED
    Inventors: Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Jeffrey Glenn Lynn
  • Publication number: 20200115666
    Abstract: An aseptic bioprocess package is provided herein. The aseptic bioprocess package includes a 2D flexible container including an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container and a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet.
    Type: Application
    Filed: March 16, 2018
    Publication date: April 16, 2020
    Inventors: Vasiliy Nikolaevich Goral, Ryann Loren Russell, JR.
  • Publication number: 20190322969
    Abstract: The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.
    Type: Application
    Filed: June 20, 2019
    Publication date: October 24, 2019
    Inventors: Ye Fang, Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Gregory Roger Martin, Kaitlyn Mary Matias, Michael Kurt Schaefer, Allison Jean Tanner
  • Publication number: 20170342363
    Abstract: The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.
    Type: Application
    Filed: April 20, 2017
    Publication date: November 30, 2017
    Inventors: Ye Fang, Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Gregory Roger Martin, Kaitlyn Mary Matias, Michael Kurt Schaefer, Allison Jean Tanner
  • Publication number: 20170253844
    Abstract: A cell culture apparatus has a structure defining a cell culture microwell. The cell culture microwell defines a top aperture and an inner surface and an axis extending through the top aperture. The top aperture defines a top diametric dimension measured perpendicular to the axis and the inner surface defines a first diametric dimension measured perpendicular to the axis at a widest portion of the microwell and a second diametric dimension measured perpendicular to the axis. The second diametric dimension is at a location along the axis farther from the top aperture than a location along the axis of the first diametric dimension. The top diametric dimension is less than the first diametric dimension, and the second diametric dimension is less than the first diametric dimension.
    Type: Application
    Filed: March 3, 2017
    Publication date: September 7, 2017
    Inventors: Ye Fang, Vasiliy Nikolaevich Goral
  • Publication number: 20170226455
    Abstract: A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.
    Type: Application
    Filed: April 27, 2017
    Publication date: August 10, 2017
    Inventors: Ye Fang, Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Allison Jean Tanner, Qi Wu
  • Publication number: 20150275167
    Abstract: An ex vivo cell culture sustained release composition, including: a mixture comprising: a sustenant; and a non-biodegradable binder; and a non-biodegradable and water insoluble encapsulant coat that encapsulates the mixture, as defined herein. Also disclosed is a method for sustainably providing a sustenant to a cell culture in aqueous media, including contacting a cell culture with the sustained release composition.
    Type: Application
    Filed: March 20, 2015
    Publication date: October 1, 2015
    Inventors: Ronald Allen Faris, Ann MeeJin Ferrie, Vasiliy Nikolaevich Goral, Gregory Roger Martin, Jin Liu
  • Publication number: 20150004686
    Abstract: A microplate for culturing cells with automatic, continuous perfusion includes a well frame and a planar substrate. The well frame and the planar substrate form a first well, a second well, and a third well. The first well is fluidly connected with the second well with a first perfusion membrane and the first well is fluidly connected with the third well with a second perfusion membrane. Methods of fabricating the microplate and methods of culturing cells are also provided.
    Type: Application
    Filed: January 31, 2013
    Publication date: January 1, 2015
    Inventors: Vasiliy Nikolaevich Goral, Fang Lai, Po Ki Yuen
  • Patent number: 8679843
    Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: March 25, 2014
    Assignee: Corning Incorporated
    Inventors: Ronald Allen Faris, Vasiliy Nikolaevich Goral, Miya Yi-Cheng Hsieh, Odessa Natalie Petzold, Po Ki Yuen
  • Publication number: 20130273589
    Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.
    Type: Application
    Filed: June 6, 2013
    Publication date: October 17, 2013
    Inventors: Ronald Allen Faris, Vasiliy Nikolaevich Goral, Miya Yi-Cheng Hsieh, Odessa Natalie Petzold, Po Ki Yuen
  • Publication number: 20130029422
    Abstract: A cell culture article comprises a substrate having a micro-structured surface and a thin hydrophobic elastomeric coating disposed on the substrate. The coating forms a micro-structured cell culture surface and is sufficiently thin to reduce absorption of hydrophobic molecules from an aqueous medium in contact with the coating, relative to articles fabricated entirely from the hydrophobic elastomer.
    Type: Application
    Filed: July 26, 2011
    Publication date: January 31, 2013
    Inventors: Vasiliy Nikolaevich Goral, Yulong Hong, Hui Su, Jian Tan
  • Publication number: 20120070878
    Abstract: A micro fluidic apparatus includes (i) a first conduit; (ii) a second conduit; and (iii) a first interconnected microporous network in communication with the first and second conduits and configured to allow diffusion of gas between the first and second conduits. The microporous network comprises poly(dimethylsiloxane) (PDMS) and prevents flow of aqueous fluid between the first and second conduits through the microporous network.
    Type: Application
    Filed: September 16, 2011
    Publication date: March 22, 2012
    Inventors: Katherine A. Fink, Vasiliy Nikolaevich Goral, Hui Su, Po Ki Yuen
  • Publication number: 20100129856
    Abstract: The disclosure provides a device for measuring cellular responses under controlled environments. The disclosure also provides an array of devices for measuring cellular responses under controlled environments. The disclosure also provides methods to study cell signaling using the devices. By using microfluidics, the disclosure enables study of cell signaling under well-defined environment conditions. Such capability can be used for differentiating long-acting ligands from short-acting ligands, for determining the kinetics of receptor resensitization and functional recovery of receptor signaling, and for differentiating the sensitivity of a cell type to laminar flow-induced stress force. Through combination with conventional static label-free cell assays, the full spectrum of a drug compound can be studied in details, thus creating a complete representation of its pharmacology acting on living cells.
    Type: Application
    Filed: November 23, 2009
    Publication date: May 27, 2010
    Inventors: Ye Fang, Vasiliy Nikolaevich Goral, Joydeep Lahiri, Deepti Jayaraman Mudaliar, Elizabeth Tran, Qi Wu