Abstract: A system for clarifying a cell culture harvest solution, including target molecules and dynamic filter media. A filtration vessel includes a flexible liner and at least one filter having a surface on which the dynamic filter media accumulates into a cake. The cake and filter during filtration operation permit a filtrate including target molecules to pass therethrough while preventing unwanted solid materials from passing therethrough. A backflush source includes a backflush fluid and is fluidly connected to the filtration vessel via the at least one filter. The backflush source, during backflush operation, is adapted to supply backflush fluid back through the at least one filter for removing the cake formed on the filter. Related systems and methods are also disclosed.

Abstract: The invention provides a method and a kit for combined virus inactivation and capture/purification of a feed containing a protein of interest. The method comprises the steps of: contacting a feed comprising a protein of interest with negatively charged particles or media, conditioning the particles or media such that the pH is acidic thereby inactivating said virus, and eluting said protein of interest.

Abstract: The current disclosure describes a method for purifying a protein, wherein said protein is present in a feed, and wherein said method comprises a multimodal chromatography step, wherein said feed is contacted with a multimodal ion exchanger comprising a ligand with a hydrophobic moiety and a charged moiety and wherein binding of said protein of interest to said exchanger occurs under high conductivity conditions.

Abstract: A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.