Abstract: The present invention lies in the technical field of enzymatic (poly)peptide ligation and specifically relates to methods that allow the ligation of (poly)peptides and oligonucleotides. The methods comprise providing at least one cargo molecule modified with a peptide tag and at least one poly(peptide) to be ligated to the cargo molecule, wherein the peptide tag and/or the (poly)peptide comprises a ligation motif for a peptide ligase, preferably sortase and peptidyl asparaginyl ligases (PALs), such as butelase-1, VyPAL2 or OaAEPI b. The invention also relates to the resulting conjugates and the corresponding uses.

Abstract: The present invention relates to conjugates comprising (a) an antisense oligonucleotide (ASO) and (b) at least one G-quadruplex structure, wherein the ASO and the at least one G-quadruplex structure are heterologous to each other. The G-quadruplex of the conjugate may be further conjugated with ligands or functional moieties for addressable delivery and enhanced properties including potency and therapeutic index. Further encompassed are such conjugates for use as a medicament, a method of modulating the stability, translation, splicing, cleavage, or activity of a target nucleic acid molecule using such conjugates, and use of G-quadruplex in stabilizing antisense oligonucleotides.

Abstract: The present invention relates to conjugates comprising (a) an antisense oligonucleotide (ASO) and (b) at least one G-quadruplex structure, wherein the ASO and the at least one G-quadruplex structure are heterologous to each other. The G-quadruplex of the conjugate may be further conjugated with ligands or functional moieties for addressable delivery and enhanced properties including potency and therapeutic index. Further encompassed are such conjugates for use as a medicament, a method of modulating the stability, translation, splicing, cleavage, or activity of a target nucleic acid molecule using such conjugates, and use of G-quadruplex in stabilizing antisense oligonucleotides.

Abstract: The present invention is directed to a method for fabricating a gold nanoparticle, the method comprising the steps of contacting a gold ion with a protein, wherein the protein has an inner cavity that can accommodate the gold ion, separating the protein with the encapsulated gold ion(s) from non-encapsulated gold ions, contacting the protein-encapsulated gold ion with a first reductant to reduce the gold ion and form a gold nanocluster seed in the inner cavity of the protein, wherein the first reductant is a strong reductant, and contacting the gold nanocluster seed in the inner cavity of the protein with a second reductant to mineralize and grow the gold nanoparticle.

Abstract: A peptide comprising or consisting of the amino acid sequence of SEQ ID NO:1 or a derivative thereof is provided. Also provided are conjugates of such a peptide, the use of the peptide or the conjugates as a medicament, and methods for the detection of parallel-stranded G-quadruplexes.

Abstract: This invention relates to a peptide comprising or consisting of the amino acid sequence of SEQ ID NO:1 or a derivative thereof. The present invention also relates to conjugates of said peptide, the use of the peptide or the conjugate as a medicament and to methods for the detection of parallel-stranded G-quadruplexes.

Abstract: The present invention is directed to a method for fabricating a gold nanoparticle, the method comprising the steps of contacting a gold ion with a protein, wherein the protein has an inner cavity that can accommodate the gold ion, separating the protein with the encapsulated gold ion(s) from non-encapsulated gold ions, contacting the protein-encapsulated gold ion with a first reductant to reduce the gold ion and form a gold nanocluster seed in the inner cavity of the protein, wherein the first reductant is a strong reductant, and contacting the gold nanocluster seed in the inner cavity of the protein with a second reductant to mineralize and grow the gold nanoparticle.