Abstract: A range of Cel7 putative cellobiohydrolase genes were identified using genome mining and homologous sequence alignment to Cel7A from Trichoderma reesei. A representative subset of these genes from across a broad diversity of evolutionarily disparate sources were cloned and expressed in T. reesei using a constitutive promotor and a common secretion signal. The purified recombinant enzymes were tested for efficacy on various substrates. The top performers were subjected to structural studies and subsites likely to confer enhanced performance were predicted using homology modeling and comparisons of natural sequence diversity. Once identified, the subsites were genetically introduced individually and combinatorically into the best in class Cel7A backbone we have found to date and then expressed in T. reesei and tested. A triple mutant was determined to have the highest cellulase activity we have measured in a cellobiohydrolase to date.

Abstract: Disclosed are various embodiments that may facilitate transferring an item to a receptacle using a conveyor and a robotic drive unit. The robotic drive unit moves to be proximate to a receptacle. The robotic drive unit can align an item with the receptacle. The item can be transferred to the receptacle via the conveyor.

Abstract: Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Abstract: Disclosed herein are chimeric Cel7A polypeptides useful for producing biofuels from lignocellulosic biomass.

Abstract: Consistent access to data objects transcending storage limitations in a non-relational data store may be provided. A data object may be stored in data chunks across separately accessible data items in a non-relational data store. Consistency indications may also be stored along with the data chunks in the data items that may be used to provide consistent access to the data object. When update to the data object is received, the data chunks and consistency indications of the data object may be retrieved and evaluated to determine if the data object is in a consistent state. If the data object is consistent, then the new versions of the data chunks and consistency indications may be generated. Authorization to update the data object may be obtained, and then the new versions stored in the data items in the non-relational data store.

Abstract: Techniques for determining an inventory level for an ordered item and offering delivery for the ordered item within a time frame may be provided. Information about a plurality of merchants may be maintained. An inventory level of the plurality of merchants for an item ordered by a customer may be determined based on the information and a threshold associated with the item. A particular merchant that is within a geographic range of the customer may be selected based on the inventory level and the merchant information. In an example, delivery instructions may be provided to a delivery service for delivering the item to the customer within the time frame.

Abstract: Disclosed are various embodiments that may facilitate transferring an item to a receptacle using a conveyor and a robotic drive unit. The robotic drive unit moves to be proximate to a receptacle. The robotic drive unit can align an item with the receptacle. The item can be transferred to the receptacle via the conveyor.

Abstract: Disclosed herein are chimeric Cel7A polypeptides useful for producing biofuels from lignocellulosic biomass.

Abstract: Disclosed are various embodiments that may facilitate transferring an item to a receptacle using a tiltable floor and a robotic drive unit. The robotic drive unit moves along a travel path to be proximate to a receptacle. The robotic drive unit can align an item with the receptacle. The item can be transferred by causing the tiltable floor to tilt.

Abstract: Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Abstract: Disclosed are various embodiments that may facilitate transferring an item to a receptacle using a tiltable floor and a robotic drive unit. The robotic drive unit moves along a travel path to be proximate to a receptacle. The robotic drive unit can align an item with the receptacle. The item can be transferred by causing the tiltable floor to tilt.

Abstract: Disclosed are various embodiments that may facilitate retrieval of items from a storage location. A robotic drive unit transports a receptacle to be proximate to a target storage unit that comprises an item. The robotic drive unit moves an item mover to transfer the item from the target storage unit to the receptacle. The robotic drive unit transports the item in the receptacle to a destination.

Abstract: Disclosed are various embodiments that may facilitate retrieval of items from a storage location. A robotic drive unit transports a receptacle to be proximate to a target storage unit that comprises an item. The robotic drive unit moves an item mover to transfer the item from the target storage unit to the receptacle. The robotic drive unit transports the item in the receptacle to a destination.

Abstract: Disclosed are various embodiments that may facilitate retrieval of items from a storage location. A robotic drive unit transports a carrier storage unit to be proximate to a target storage unit that comprises a container in which an item is located. The robotic drive unit moves a container mover to transfer the container from the target storage unit to the carrier storage unit. The robotic drive unit transports the carrier storage unit with the container to a destination.

Abstract: Embodiments herein are directed to an inventory system having multiple inventory holders and drive units for moving the inventory holders. Features herein are directed to moving inventory items relative to a bin of an inventory holder or between inventory holders in the inventory system. The inventory system may include actuation units or some other mechanism on the drive units for assisting in moving inventory items relative to a bin of an inventory holder or between inventory holders. The inventory holders each include one or more inventory bins. A mobile drive unit is included, which includes an actuator configured for selectively actuating at least one of the inventory bins for moving at least one inventory item relative to the bin.

Abstract: Disclosed are various embodiments that may facilitate retrieval of items from a storage location. A robotic drive unit transports a carrier storage unit to be proximate to a target storage unit that comprises a container in which an item is located. The robotic drive unit moves a container mover to transfer the container from the target storage unit to the carrier storage unit. The robotic drive unit transports the carrier storage unit with the container to a destination.

Abstract: Disclosed are various embodiments that may facilitate items being retrieved from a storage location. A robotic drive unit transports a carrier storage unit to be proximate to a target storage unit that comprises a container in which an item is located. The robotic drive unit aligns a receptacle in the carrier storage unit with the container in the target storage unit. The container is transferred from the target storage unit to the carrier storage unit. The robotic drive unit transports the carrier storage unit with the container to a destination.

Abstract: Embodiments herein are directed to an inventory system having multiple inventory holders and drive units for moving the inventory holders. Features herein are directed to moving inventory items relative to a bin of an inventory holder or between inventory holders in the inventory system. The inventory system may include actuation units or some other mechanism on the drive units for assisting in moving inventory items relative to a bin of an inventory holder or between inventory holders. The inventory holders each include one or more inventory bins. A mobile drive unit is included, which includes an actuator configured for selectively actuating at least one of the inventory bins for moving at least one inventory item relative to the bin.

Abstract: Disclosed are various embodiments that may facilitate items being retrieved from a storage location. A robotic drive unit transports a carrier storage unit to be proximate to a target storage unit that comprises a container in which an item is located. The robotic drive unit aligns a receptacle in the carrier storage unit with the container in the target storage unit. The container is transferred from the target storage unit to the carrier storage unit. The robotic drive unit transports the carrier storage unit with the container to a destination.