Abstract: Cell based sensor. The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a non-protease hydrolase as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis. Said granule stored non protease-hydrolase reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific substrate; absence of toxicity when cell thawing; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.

Abstract: The present invention surprisingly shows that the addition of a heterologous viral-GPCR (G protein-coupled receptor) derived sequence at the amino terminal end of GPCRs improves cell surface expression of such receptors in eukaryotic cells. Transfected cells expressing the above heterologous viral GPCRs on their surface are useful for cell based assays to identify test compounds that increases or modulate activity of a GPCR or a ligand of a GPCR for example for drug discovery, development of novel flavors in the food industry or development of sensors based on odor GPCRs. In addition, membrane extracts derived from transfected cells may be used for example for assays of compounds in drug discovery or for development of sensors comprising such membrane extracts for identification and/or quantification of volatile organic compounds.

Abstract: The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.

Abstract: Cell based sensor. The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a non-protease hydrolase as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis. Said granule stored non protease-hydrolase reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific substrate; absence of toxicity when cell thawing; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.

Abstract: The present invention surprisingly shows that the addition of a heterologous viral-GPCR (G protein-coupled receptor) derived sequence at the amino terminal end of GPCRs improves cell surface expression of such receptors in eukaryotic cells. Transfected cells expressing the above heterologous viral GPCRs on their surface are useful for cell based assays to identify test compounds that increases or modulate activity of a GPCR or a ligand of a GPCR for example for drug discovery, development of novel flavors in the food industry or development of sensors based on odor GPCRs. In addition, membrane extracts derived from transfected cells may be used for example for assays of compounds in drug discovery or for development of sensors comprising such membrane extracts for identification and/or quantification of volatile organic compounds.

Abstract: The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.