Abstract: A bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein and compositions thereof are provided. Also provided are recombinant host cells for producing the bioconjugate, and methods of producing the bioconjugate using the recombinant host cells. The recombinant host cells contain a nucleic acid encoding a glucosyl transferase capable of modifying the E. coli O4 antigen with glucose branching to produce the glucosylated O4 antigen polysaccharide. Bioconjugates of an E. coli glucosylated O4 antigen polysaccharide described herein can be used alone or in combination with one or more additional E. coli O-antigen polysaccharides to induce antibodies against an E. coli glucosylated antigen, and to vaccinate a subject against extra-intestinal pathogenic E. coli (ExPEC).

Abstract: A portable charging station including a housing, a handle coupled to the housing and extending along a height axis of the portable charging station, a charging circuit disposed in the housing, a power tool battery pack interface electrically connected to the charging circuit, and a door including a hinge connected to the housing. The charging circuit includes a rechargeable battery. The power tool battery pack interface defines an insertion axis. The insertion axis extends along the height axis. The door is pivotable between an open position and a closed position. The door is intersected by the insertion axis in the closed position.

Abstract: A tutorial for a given application may be leveraged to generate executable code that can then be executed within a native instruction service of the application. In this way, a software application may thus provide an integrated, interactive learning experience for a user, in a manner that extends beyond the instructional content included in the native instruction service, i.e., that includes at least a portion of the instructional content of the tutorial.

Abstract: A tutorial for a given application may be leveraged to generate executable code that can then be executed within a native instruction service of the application. In this way, a software application may thus provide an integrated, interactive learning experience for a user, in a manner that extends beyond the instructional content included in the native instruction service, i.e., that includes at least a portion of the instructional content of the tutorial.