Abstract: The present invention is related to a method and kit (or device) for the detection and/or the quantification of biomarkers related to tumorigenesis. Said method is advantageously used to propose or adapt an anti-tumoral therapeutic protocol to be administered to a subject. Furthermore, said method and kit (or device) are a technical platform for the identification of new compounds, which are preferably used at (a) specific step(s) of an anti-tumoral therapeutic protocol.

Abstract: The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.

Abstract: A method quantifies a target compound selected from the group consisting of a polynucleotide or a protein present in a sample solution. The method includes putting into contact a target compound with a capture probe and detecting signals resulting from the binding between the target compound and its corresponding capture probe and resulting from the printed detection molecule in the different discrete regions. The method obtains a detection curve of the detected signals of the detection molecule and converts the signal obtained from the target compound bound to a specific capture probe into a concentration value and quantifies the target compound by converting the concentration value into a target amount using a target concentration curve.

Abstract: This invention provides a method for evaluation of the level of activation of cells through the quantification of the phosphorylated versus the non-phosphorylated multiple proteins belonging to kinase cascades which is the result of the equilibrium between the kinase/phosphatase activities in the given cells. The invention also provides a method for quantification of the level of phosphorylation of enzymes and proteins involved into the pathways of cell activation or being the products of theses activations. In particular, the invention provides a method for the simultaneous quantification level of phosphorylation of multiple proteins of the same or different pathways present in the same cell extract.

Abstract: This invention provides a method for evaluation of the level of activation of cells through the quantification of the phosphorylated versus the non-phosphorylated multiple proteins belonging to kinase cascades which is the result of the equilibrium between the kinase/phosphatase activities in the given cells. The invention also provides a method for quantification of the level of phosphorylation of enzymes and proteins involved into the pathways of cell activation or being the products of theses activations. In particular, the invention provides a method for the simultaneous quantification level of phosphorylation of multiple proteins of the same or different pathways present in the same cell extract.