Abstract: The present invention refers to an expression vector for gram-negative bacteria allowing for the production of hybrid proteins between single domain recombinant antibodies and a transporter domain of an autotransporter, as well as to its anchoring and expression on the external surface of a bacterial membrane, and to a method of specific bacterial adhesion to a surface containing a specific antigen.

Abstract: The system comprises a DNA construct comprising: a) a first nucleic acid sequence containing the nucleotide sequence coding for a product of interest; b) a second nucleic acid sequence containing the nucleotide sequence coding for a dimerization domain; and c) a third nucleic acid sequence containing the nucleotide sequence coding for Escherichia coli ?-hemolysin (HlyA) or for a fragment of said protein comprising the recognition signal of the E. coli hemolysin (Hly) transport system secretion mechanism. It is applicable in producing recombinant dimeric proteins.

Abstract: The present invention relates to the design, preparation and use of cascade regulatory circuits for amplification of gene expression. The genetic circuit is based on a plurality (e.g., two or more) of regulatory genes organized in a hierarchical order of expression in a genetic construct or constructs, which can be established in a cell, e.g., a gram-negative bacteria, by means of autoreplicative vectors or by chromosomal insertion. In one embodiment, the genetic construct(s) can be stabily maintained in the chromosome without selective pressure, and gene expression induced three orders of magnitude therefrom using economical biodegradable benzoate derivatives.

Abstract: The present invention relates to the design, preparation and use of cascade regulatory circuits for amplification of gene expression. The genetic circuit is based on a plurality (e.g., two or more) of regulatory genes organized in a hierarchical order of expression in a genetic construct or constructs, which can be established in a cell, e.g., a gram-negative bacteria, by means of autoreplicative vectors or by chromosomal insertion. In one embodiment, the genetic construct(s) can be stabily maintained in the chromosome without selective pressure, and gene expression induced three orders of magnitude therefrom using economical biodegradable benzoate derivatives.