Abstract: The methodology shown in this invention is suitable for the preparation of meat substitute food products of animal origin (e.g., beef, pork, poultry, deer, or fish), with nutritional characteristics, texture, flavor, and odor very similar to the meat subject matter of the substitution, containing at least one of the following meats: beef, pork, poultry, or fish, or a combination thereof, and added with vegetable protein.

Abstract: A method for multiple and simultaneously detecting and quantifying of Listeria spp, Staphylococcus aureus, Campylobacter jejuni, and Escherichia coli 0157:H7, by extracting DNA from a sample; preparing a reaction mixture for enzymatic amplification of the extracted DNA and sets of pairs of oligonucleotide primers identified as SEQ ID No. 1 and SEQ ID No. 2, SEQ ID No. 4 and SEQ ID No. 5, SEQ ID No. 7 and SEQ ID No. 8, and SEQ ID No. 10 and SEQ ID No. 11, and probes with oligonucleotide sequences identified as SEQ ID No. 3, SEQ ID No. 6, SEQ ID No. 9 and SEQ ID No. 12; providing a thermostable DNA polymerase and magnesium salt to the reaction mixture; amplifying the reaction mixture by a PCR reaction; and determining the presence or absence, and quantification of the pathogens by using a fluorescent signal or fluorescence emission specific for each pathogen.

Abstract: A composition for promoting control of total and LDL cholesterol and/or weight loss and/or thermogenesis in individuals, the composition including green tea extract in a quantity of about 200 mg to about 300 mg; guarana extract in a quantity of about 160 mg to about 260 mg; yerba mate extract in a quantity of about 100 mg to about 300 mg; and conjugated linoleic acid in a quantity of about 700 mg to 3400 mg. Additionally, the green tea extract is standardized in a content of at least 50% epigallocatechin gallate (EGCG), while the conjugated linoleic acid is standardized in a content of at least 50% of trans10, cis 12-CLA (10-12 CLA), and 50% cis9, trans11-CLA (9-11 CLA).

Abstract: A bacterial strain of human origin belonging to the species Bifidobacterium animalis identified as PTA-9175 which produces between 120 ng/ml to 398 ng/ml of folic acid and which can be used as a probiotic in food compositions. The Bifidobacterium animalis strain is deposited with the American Type Culture Collection (ATCC) in the United States of America, under the Budapest Treaty, dated on Apr. 23, 2008, and respectively identified with the ATCC deposit designation number for patent as PTA-9175.

Abstract: A method for the detection and multiple, simultaneous quantification of any combination of Listeria sp., Staphylococcus aureus, Campylobacter jejuni and/or Escherichia coli O157.-H7, in one or more test samples, by multiplex amplification reaction, using real-time polymerase chain reaction (PCR). The steps in the method are: (a) extracting DNA from the test sample or samples; (b) preparing a reaction mixture specific for the pathogens to be detected and quantified, such that the reaction mixture contains the necessary reagents for enzymatic amplification of the extracted DNA and identification of the pathogens to be detected and quantified; (c) amplifying the reaction mixture by means of multiplex amplification reaction using PCR; and (d) simultaneously determining the presence or absence of and quantification of the pathogens in the test sample or samples.