Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract: Provided herein is a ECSP computer device for facilitating senior user engagement. The processor is programmed to: (i) store user registration information for a senior user associated with the first client device; (ii) store caregiver registration information for a plurality of caregivers associated with the senior user, wherein the caregiver registration information includes data for identifying the at least one second client device; (iii) receive, from at least one of the senior user and the plurality of caregivers, one or more items of an order of the senior user; (iv) compile the received items into a list; (v) generate the order based on user preferences and the list; (vi) determine at least one third party to fulfill the order based on the items on the list and the user preferences; and (vii) transmit the order to a third party server associated with the determined third party.