Abstract: A biologically functional polynucleotide in the form of therapeutic exogenous mRNA having hairpins in lieu of a cap and poly(A) tail and method of producing the same is provided. The design of a biologically functional polynucleotide allows for a one step method of production. The target mRNA vector or chemically synthesized mRNA molecule of the biologically functional polynucleotide includes internal translation initiation site and delivered gene sequence. Additionally, the target mRNA vector is designed to form stable hairpins at both the 5? end and 3? end, wherein said hairpins comprise at least four paired nucleotides in the stem and linked to the closest end with no more than two unpaired nucleotides.