Abstract: A measurement device includes mechanical support elements (101-104) for supporting a sample well, other mechanical support elements (105-109) for supporting a measurement head (112) suitable for optical measurements, and a control system (111) configured to control the measurement head to carry out at least two optical measurements from at least two different measurement locations inside the sample well, where each measurement location is a center point of a capture range from which radiation is captured in the respective optical measurement. The final measurement result is formed from the results of the at least two optical measurements in accordance with a pre-determined rule. The use of the at least two optical measurements from different measurement locations reduces measurement variation in situations where the sample well (153) contains a piece (158) of sample carrier.

Abstract: A measurement device includes mechanical support elements (101-104) for supporting a sample well, other mechanical support elements (105-109) for supporting a measurement head (112) suitable for optical measurements, and a control system (111) configured to control the measurement head to carry out at least two optical measurements from at least two different measurement locations inside the sample well, where each measurement location is a center point of a capture range from which radiation is captured in the respective optical measurement. The final measurement result is formed from the results of the at least two optical measurements in accordance with a pre-determined rule. The use of the at least two optical measurements from different measurement locations reduces measurement variation in situations where the sample well (153) contains a piece (158) of sample carrier.

Abstract: A measurement device includes mechanical support elements (101-104) for supporting a sample well, other mechanical support elements (105-109) for supporting a measurement head (112) suitable for optical measurements, and a control system (111) configured to control the measurement head to carry out at least two optical measurements from at least two different measurement locations inside the sample well, where each measurement location is a center point of a capture range from which radiation is captured in the respective optical measurement. The final measurement result is formed from the results of the at least two optical measurements in accordance with a pre-determined rule. The use of the at least two optical measurements from different measurement locations reduces measurement variation in situations where the sample well (153) contains a piece (158) of sample carrier.

Abstract: A measurement device includes mechanical support elements (101-104) for supporting a sample well, other mechanical support elements (105-109) for supporting a measurement head (112) suitable for optical measurements, and a control system (111) configured to control the measurement head to carry out at least two optical measurements from at least two different measurement locations inside the sample well, where each measurement location is a center point of a capture range from which radiation is captured in the respective optical measurement. The final measurement result is formed from the results of the at least two optical measurements in accordance with a pre-determined rule. The use of the at least two optical measurements from different measurement locations reduces measurement variation in situations where the sample well (153) contains a piece (158) of sample carrier.

Abstract: The invention relates to a method for improving the detection sensitivity in homogenous TR-FRET based bioaffinity assays. The sensitivity is improved by the use of long lifetime donors together with a high energy transfer efficiency and by carrying out the detection of the energy transfer based emission of the acceptor in a time window which is opened after a delay of 1 microsecond or more, but less than 50 microseconds, calculated from the donor excitation, and which time window has a width of 1 microsecond or more, but less than 100 microseconds. The invention concerns also the use of the improved method in multianalyte assays. Furthermore, the invention concerns a device suitable for carrying out the improved method.

Abstract: The present technology relates generally to fibre optic cables their manufacture and uses in the field of optical measurements including biochemical laboratory instrumentation for measuring properties of samples on microtitration plates and corresponding sample supports. The technology has also applications in various laser technologies. A fibre optic cable has an active surface with a determined form provided at a first optical interface at the first end of the cable. The first end of the cable is fused into an exemplary circular form, the fused cable end including fibre ends both within the active surface and outside the active surface. At the opposite, second end of the cable, those fibres which have their first ends at the determined active surface area, are used for forming a second optical interface. It is possible to have high transmission efficiency in optical interfaces where other than circular cross section of the light beam exists.

Abstract: The present technology relates generally to fibre optic cables their manufacture and uses. The technology has useful applications e.g. in the field of optical measurements such as biochemical laboratory instrumentation for measuring properties of samples on microtitration plates and corresponding sample supports. The technology has also applications in various laser technologies. The object of the technology is achieved by providing a fibre optic cable wherein an active surface with a determined form is provided at a first optical interface at the first end (451a, 451b) of the cable. The first end of the cable is fused into an exemplary circular form, the fused cable end including fibre ends both within the active surface and outside the active surface. At the opposite, second end of the cable, those fibres which have their first ends at the determined active surface area, are used for forming a second optical interface (452a, 452b).

Abstract: The invention concerns a labeled probe for use in bioaffinity assays based on time-resolved fluorescence resonance energy transfer, wherein said probe comprises an energy donor as well as an energy acceptor, and the energy transfer signal of the acceptor is to be measured. The invention concerns also a homogeneous time-resolved fluorescence resonance energy transfer bioaffinity assay based on the use of said probe.

Abstract: This invention relates to a novel time-resolved lanthanide-based energy transfer assay utilizing non-overlapping acceptor fluorophores, having their absorption maximum energetically at higher level than the main emittive energy level of the donor. In this assay the lifetime of the energy transfer enhanced acceptor signal is partially independent of the energy transfer rate. The use of non-overlapping acceptors enables an anti-Stokes' shift FRET measurement, in which the acceptor emission is created and measured at a shorter wavelength than the actual donor emission.

Abstract: The invention relates to a method for improving the detection sensitivity in homogenous TR-FRET based bioaffinity assays. The sensitivity is improved by the use of long lifetime donors together with a high energy transfer efficiency and by carrying out the detection of the energy transfer based emission of the acceptor in a time window which is opened after a delay of 1 microsecond or more, but less than 50 microseconds, calculated from the donor excitation, and which time window has a width of 1 microsecond or more, but less than 100 microseconds. The invention concerns also the use of the improved method in multianalyte assays. Furthermore, the invention concerns a device suitable for carrying out the improved method.